##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512779_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1497826 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.80623316727043 31.0 25.0 34.0 10.0 34.0 2 28.337035810568118 31.0 27.0 34.0 16.0 34.0 3 29.46072774808289 31.0 30.0 34.0 22.0 34.0 4 33.29531667897339 35.0 35.0 37.0 28.0 37.0 5 33.65261786081962 37.0 35.0 37.0 28.0 37.0 6 33.9437377906379 37.0 35.0 37.0 30.0 37.0 7 33.926161650285145 37.0 35.0 37.0 30.0 37.0 8 33.96614893852824 37.0 35.0 37.0 30.0 37.0 9 35.52735297691454 39.0 37.0 39.0 30.0 39.0 10 35.44013189783059 39.0 37.0 39.0 30.0 39.0 11 35.49433245250116 39.0 37.0 39.0 30.0 39.0 12 35.39717230172263 39.0 37.0 39.0 30.0 39.0 13 35.390094042966275 39.0 37.0 39.0 30.0 39.0 14 36.56855402429922 40.0 37.0 41.0 30.0 41.0 15 36.48088896841155 40.0 37.0 41.0 30.0 41.0 16 36.38140945610505 40.0 37.0 41.0 29.0 41.0 17 36.335330672588135 40.0 37.0 41.0 29.0 41.0 18 36.24513595037074 40.0 37.0 41.0 28.0 41.0 19 36.26398660458558 40.0 37.0 41.0 28.0 41.0 20 36.181728051188855 40.0 37.0 41.0 27.0 41.0 21 36.08491440260751 40.0 37.0 41.0 27.0 41.0 22 35.96192014292715 40.0 37.0 41.0 27.0 41.0 23 35.81368730413279 40.0 37.0 41.0 27.0 41.0 24 35.69582581688394 40.0 37.0 41.0 27.0 41.0 25 35.57532784181874 40.0 36.0 41.0 26.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 3.6783970078005943 1101 2 2.1344808000719446 1101 3 1.1783828309473812 1101 4 1.0689696866699876 1101 5 0.6826656402928677 1101 6 0.3859136231658127 1101 7 0.23045151915589202 1101 8 0.19724641413081656 1101 9 0.1643219122124151 1101 10 0.10400944179366434 1101 11 0.09314751828220835 1101 12 0.08458617655634981 1101 13 -0.010048861217079263 1101 14 -0.1704509432545862 1101 15 -0.2399684142695051 1101 16 -0.3802204333664534 1101 17 -0.33929004635312765 1101 18 -0.24003245508954052 1101 19 -0.3055018033793431 1101 20 -0.4664993217174569 1101 21 -0.499394696901291 1101 22 -0.40472164172557257 1101 23 -0.3384586904330362 1101 24 -0.39925840268890056 1101 25 -0.4970653001424452 1102 1 3.93064600322613 1102 2 2.4824598326215472 1102 3 1.3841085577865506 1102 4 1.1289799757371597 1102 5 0.8379749379945096 1102 6 0.7944869188652603 1102 7 0.6974778397020458 1102 8 0.6396206859012352 1102 9 0.5399423846752569 1102 10 0.47587579515360545 1102 11 0.31846418518060915 1102 12 0.3126419599853847 1102 13 0.2984145917382435 1102 14 0.26848900474390547 1102 15 0.2631391620990158 1102 16 0.2112201750789282 1102 17 0.15956286169875966 1102 18 0.18903555008280648 1102 19 0.20497496974672202 1102 20 0.28089017509154246 1102 21 0.09724606170885153 1102 22 0.17794545133487105 1102 23 -0.008304771549816792 1102 24 0.14027803312261966 1102 25 0.060629948811268264 1103 1 2.8071434068695957 1103 2 1.5146599965382883 1103 3 0.6133848341142922 1103 4 -0.0026095831897023913 1103 5 -0.17867480942652492 1103 6 -0.3521737059337511 1103 7 -0.4391920293560929 1103 8 -0.5406579927241637 1103 9 -0.8468885280466409 1103 10 -0.7790953883635936 1103 11 -0.441511197064969 1103 12 -0.607449635803583 1103 13 -0.6649234023684159 1103 14 -1.185231733950019 1103 15 -1.1064099257234616 1103 16 -0.9885238688257587 1103 17 -0.9452732870844116 1103 18 -1.095666965222648 1103 19 -1.1391413163797282 1103 20 -1.1009516603727363 1103 21 -1.1545727992008352 1103 22 -1.261275813082193 1103 23 -1.493420203817223 1103 24 -1.409039131662098 1103 25 -1.2815150015751868 1104 1 -6.536681773563441 1104 2 -8.12244371299808 1104 3 -7.710191749686832 1104 4 -6.16169183307812 1104 5 -3.9152752296205087 1104 6 -2.585752394333422 1104 7 -2.2565736547593254 1104 8 -1.8520787056024943 1104 9 -1.9752563393004365 1104 10 -1.6777489895857087 1104 11 -1.4844958080952466 1104 12 -1.4434415367277964 1104 13 -1.5955251724083652 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-0.025170866726355712 2316 6 -0.24383008487784252 2316 7 -0.3058631447676632 2316 8 -0.39399955623555627 2316 9 -0.4073024837593877 2316 10 -0.42387423013285286 2316 11 -0.4724021694493885 2316 12 -0.30784334972704386 2316 13 -0.31709272159923074 2316 14 -0.18653299183264238 2316 15 -0.1596892068062914 2316 16 -0.16540040503298314 2316 17 -0.17918829381314083 2316 18 -0.15584180089712873 2316 19 -0.05382551110129441 2316 20 -0.3270646807368678 2316 21 -0.18102058761853357 2316 22 -0.1964019906093526 2316 23 -0.13397829886465473 2316 24 -0.19295803104261466 2316 25 -0.19608241674318094 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 55344.0 3 3.0 4 455.0 5 2038.0 6 2452.0 7 2287.0 8 1997.0 9 2012.0 10 1983.0 11 1974.0 12 2033.0 13 2037.0 14 2002.0 15 2072.0 16 2153.0 17 2312.0 18 2556.0 19 3049.0 20 3821.0 21 4298.0 22 5437.0 23 6300.0 24 7462.0 25 8347.0 26 9569.0 27 11196.0 28 13195.0 29 15618.0 30 19823.0 31 26027.0 32 34349.0 33 48080.0 34 69985.0 35 104012.0 36 181055.0 37 257388.0 38 583105.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.08404662069284 20.29221849186013 11.629896439984318 23.99383844746271 2 15.086950350051156 19.98063603786016 40.74078537873553 24.191628233353153 3 18.93123219144332 24.38895226771215 29.497731752254857 27.18208378858967 4 12.546105449498421 16.577340322324712 37.87419145988817 33.00236276828869 5 13.554682923073319 37.4627820014529 35.407832936631486 13.574702138842301 6 32.305469045317246 36.37140936050071 17.985461819714523 13.33765977446753 7 28.337018534651286 31.32655846459558 22.071840970988514 18.26458202976462 8 25.580688748689113 36.07086520900244 20.41517396736886 17.933272074939584 9 26.82021587797782 15.113867202659264 19.824321467092393 38.24159545227052 10 15.59520472757509 28.33635139465204 34.12540998027278 21.943033897500083 11 34.62570602564446 22.19597398078004 24.29879272960891 18.87952726396659 12 23.786470790575784 24.27781175925765 31.242780794470644 20.692936655695927 13 29.214770630350728 21.55403383904267 25.514282100004483 23.71691343060212 14 21.514510460251046 21.6209539748954 27.257640167364016 29.60689539748954 15 23.52521068418797 29.959972183718893 23.268708092002953 23.246109040090182 16 22.787930045005588 27.583693277353227 27.139007192168158 22.48936948547303 17 22.3747691101613 27.988681028372604 27.325208337807943 22.311341523658154 18 22.292325629023214 27.020791865833367 28.93640631211759 21.75047619302583 19 24.00141305213239 26.437991298221576 27.222931052824194 22.33766459682184 20 24.045155497990965 26.759061405750543 27.61720051433488 21.578582581923616 21 23.61555555257895 26.152046109973305 26.970932124951275 23.26146621249647 22 23.81857009268166 27.153662921709586 26.91974824028102 22.108018745327733 23 22.973790295543118 27.09916344588415 27.229112297801468 22.697933960771273 24 23.591305635179395 26.418307282438235 27.73752112664734 22.252865955735025 25 23.365201712838854 26.575200017688527 27.495807350520234 22.563790918952385 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1419.0 1 1419.0 2 1208.5 3 998.0 4 998.0 5 998.0 6 1628.0 7 2258.0 8 2258.0 9 2258.0 10 2832.0 11 3406.0 12 3406.0 13 3406.0 14 4977.5 15 6549.0 16 6549.0 17 6549.0 18 11492.5 19 16436.0 20 16436.0 21 16436.0 22 25491.5 23 34547.0 24 34547.0 25 34547.0 26 49671.5 27 64796.0 28 64796.0 29 64796.0 30 83652.5 31 102509.0 32 102509.0 33 102509.0 34 116910.5 35 131312.0 36 131312.0 37 131312.0 38 144100.5 39 156889.0 40 156889.0 41 156889.0 42 168276.5 43 179664.0 44 179664.0 45 179664.0 46 187774.5 47 195885.0 48 195885.0 49 195885.0 50 191844.5 51 187804.0 52 187804.0 53 187804.0 54 170587.5 55 153371.0 56 153371.0 57 153371.0 58 132596.5 59 111822.0 60 111822.0 61 111822.0 62 92530.5 63 73239.0 64 73239.0 65 73239.0 66 57308.0 67 41377.0 68 41377.0 69 41377.0 70 30719.0 71 20061.0 72 20061.0 73 20061.0 74 14219.5 75 8378.0 76 8378.0 77 8378.0 78 5827.5 79 3277.0 80 3277.0 81 3277.0 82 2227.0 83 1177.0 84 1177.0 85 1177.0 86 781.0 87 385.0 88 385.0 89 385.0 90 265.5 91 146.0 92 146.0 93 146.0 94 108.5 95 71.0 96 71.0 97 71.0 98 60.5 99 50.0 100 50.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.7437940054452254 2 0.28908564813269366 3 0.27786939203886163 4 0.283010176081868 5 0.28447897152272694 6 0.2097039308971803 7 0.44911758775718946 8 0.240949215729998 9 0.21885052068798377 10 0.19608419135467 11 0.23674311969481104 12 0.2368766465530709 13 0.25056314952471115 14 0.2721277371336857 15 0.44150655683637485 16 0.5348418307600482 17 0.5299681004335618 18 0.24595647291474443 19 0.212908575495418 20 0.20563136172025323 21 0.313788116910776 22 0.33208129649238294 23 0.43195938647079163 24 0.33668797310234966 25 0.35604936755003586 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1497826.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.9358349571602 #Duplication Level Percentage of deduplicated Percentage of total 1 85.95140708477699 65.26791862725317 2 9.744541492160964 14.799197889638691 3 2.2827666813903242 5.200313818912799 4 0.8231630534987199 2.500302950932433 5 0.3985055059032454 1.5130424162894234 6 0.22883360685339968 1.0426002601602864 7 0.13401753165237013 0.712371321544426 8 0.09408003479192131 0.571523679577858 9 0.06124768434299468 0.41858046448001 >10 0.253141084566133 3.2302521121376206 >50 0.014155566341835795 0.7662234215224906 >100 0.011842945803357922 1.9392781929965754 >500 0.0015907347121991086 0.9125298138682656 >1k 7.069932054218261E-4 1.1258650306859752 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3700 0.24702468778082368 No Hit GGTATCAACGCAGAGTACTTTTTTT 2293 0.1530885429949807 No Hit GTCCTACAGTGGACATTTCTAAATT 2038 0.13606386856684285 No Hit TATCAACGCAGAGTACTTTTTTTTT 1971 0.13159071881513607 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1966 0.1312569016694863 No Hit CTGTAGGACGTGGAATATGGCAAGA 1929 0.12878665479167806 No Hit CTTTAGGACGTGAAATATGGCGAGG 1502 0.10027867055318843 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 6.676342912995234E-5 7 0.0 0.0 0.0 0.0 6.676342912995234E-5 8 0.0 0.0 0.0 0.0 6.676342912995234E-5 9 0.0 0.0 0.0 0.0 6.676342912995234E-5 10 0.0 0.0 0.0 6.676342912995234E-5 6.676342912995234E-5 11 0.0 0.0 0.0 6.676342912995234E-5 6.676342912995234E-5 12 0.0 0.0 0.0 6.676342912995234E-5 6.676342912995234E-5 13 0.0 0.0 0.0 6.676342912995234E-5 6.676342912995234E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATAAC 85 3.405632E-6 12.456202 1 TAGGACC 335 0.0 11.054228 4 TTAGGAC 845 0.0 10.563882 3 TAGAAAT 380 0.0 10.494803 4 AGGACGT 1360 0.0 10.473107 5 AGCACCG 100 2.4104293E-5 10.445179 5 TTATACC 100 2.4113227E-5 10.444828 4 ATTACAC 175 2.3646862E-10 10.31022 3 GGACGTG 1345 0.0 10.236911 6 GTCTTAG 160 4.973117E-9 10.226825 1 GTGTAGA 255 0.0 10.191438 1 GGCGAGG 560 0.0 10.172168 19 GTCTTAT 95 1.4400935E-4 10.131839 1 TAGGCCC 75 0.002658354 10.128318 4 GTATAAA 210 5.456968E-12 10.083592 1 GGTATCA 1175 0.0 10.07579 1 GACGTGA 720 0.0 9.891269 7 GTTCTAA 185 5.11136E-10 9.885388 1 GTAGCAC 165 1.027729E-8 9.784019 3 TTACTCT 175 2.59206E-9 9.766593 4 >>END_MODULE