FastQCFastQC Report
Thu 26 May 2016
SRR1512779_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512779_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1497826
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT40020.26718724337806926No Hit
GGTATCAACGCAGAGTACTTTTTTT24930.16644122882097118No Hit
TATCAACGCAGAGTACTTTTTTTTT23410.15629318759321845No Hit
CTGTAGGACGTGGAATATGGCAAGA21770.14534398521590625No Hit
GTCCTACAGTGGACATTTCTAAATT21610.144275770349827No Hit
GTCCTAAAGTGTGTATTTCTCATTT20420.1363309222833627No Hit
CTTTAGGACGTGAAATATGGCGAGG18720.1249811393312708No Hit
GTACATGGGAAGCAGTGGTATCAAC15330.10234833685621694No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGAC1501.8189894E-1212.6742433
CTATTAG1301.9281288E-1012.4454831
TATACTG2050.012.0567925
TCCAACG752.0828111E-411.3946318
CCAACGA600.005896124511.07811419
TAGGACC4000.010.9315354
GCCTCGA803.78695E-410.682466516
GGTATCA12700.010.2665151
GCACCGT750.002650487210.1319446
GTCCTAC11800.010.081711
TGTAGGA11600.010.0809862
GGCGTGC856.600958E-410.0574458
CTCGAAC951.6515641E-49.9952918
AGCACCG1054.0740604E-59.9590015
GTAGGAC11200.09.9300433
CTACCCT1151.0187916E-59.9189724
CTAACAC1356.3866537E-79.8577443
CTGTAGG11900.09.6770871
GAACAGT2750.09.6714016
GTCCTAA7550.09.5801621
Produced by FastQC (version 0.11.5)