FastQCFastQC Report
Thu 26 May 2016
SRR1512778_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512778_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1880208
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT42000.22337954098695464No Hit
GTCCTACAGTGGACATTTCTAAATT37440.19912690510837097No Hit
GTCCTAAAGTGTGTATTTCTCATTT33950.180565128964455No Hit
CTGTAGGACGTGGAATATGGCAAGA31060.16519448912035264No Hit
CTTTAGGACGTGAAATATGGCGAGG27980.1488133227813093No Hit
GTACATGGGAAGCAGTGGTATCAAC26830.1426969782066665No Hit
GGTATCAACGCAGAGTACTTTTTTT25710.13674019044701438No Hit
TATCAACGCAGAGTACTTTTTTTTT23720.12615625505263248No Hit
GTCCTACAGTGTGCATTTCTCATTT21100.11222162654344625No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCCGA551.9698546E-413.8104176
TAGGACC8050.012.5022884
GTATAGG1251.1550583E-912.3159531
CTAGGAC2850.011.9951833
GTATAAC1501.4188117E-1011.5462061
GGCGAGG8950.011.24387219
AAGGCGT951.3689927E-510.9938196
TGTAGGA21000.010.9432252
GACAGCG1158.879233E-710.7333847
AGGACGT19400.010.5713775
GGACGTG19100.010.4888686
TATACTG2450.010.4635065
GTTAACC1002.4097377E-510.4458053
ACCGTGC1002.5016587E-510.4104258
GTAGGAC21450.010.2266633
GTTCAGG3400.010.1878281
TAAGACT2250.010.1276394
TTAGGAC12850.010.0504393
TTAGTAC951.6509008E-49.9959853
GTGTAGG4450.09.946171
Produced by FastQC (version 0.11.5)