FastQCFastQC Report
Thu 26 May 2016
SRR1512778_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512778_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1880208
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT48320.2569928433449916No Hit
GTCCTACAGTGGACATTTCTAAATT39470.20992358292274046No Hit
GTCCTAAAGTGTGTATTTCTCATTT36480.19402108702866916No Hit
CTGTAGGACGTGGAATATGGCAAGA36290.1930105605337282No Hit
CTTTAGGACGTGAAATATGGCGAGG33880.18019282972947673No Hit
GGTATCAACGCAGAGTACTTTTTTT30330.16131193995557938No Hit
GTACATGGGAAGCAGTGGTATCAAC29180.15519559538093658No Hit
TATCAACGCAGAGTACTTTTTTTTT28150.1497174780662565No Hit
GTCCTACAGTGTGCATTTCTCATTT23400.12445431569273187No Hit
CCCATGTACTCTGCGTTGATACCAC21280.11317896743339034No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGCA250.006053307618.98869716
TGTACGA400.00525115714.2616262
TAGGACC6600.012.5312764
CCGTCCA701.0925907E-412.2125429
CTAAGAC1950.011.2127773
TGGACCG600.005845502511.0911935
TACGGTG700.001482856610.8648425
GGCGAGG9400.010.80768519
GTCCTAA11050.010.6795711
AGGACCT14750.010.6346565
ATAATAC1803.45608E-1110.5627613
TGGCGAG22550.010.44195618
AAATGTC4800.010.2901967
GGACGTG20650.010.2576156
AGGACGT20950.010.2554785
GTCCTAT1951.4551915E-1110.2489431
GTAGGAC21350.010.24117853
TGTAGGA22200.010.2358222
GTATTAT2151.8189894E-1210.1808431
AATGTCC5650.010.0870558
Produced by FastQC (version 0.11.5)