FastQCFastQC Report
Thu 26 May 2016
SRR1512777_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512777_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1711515
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT39080.22833571426484722No Hit
GTCCTACAGTGGACATTTCTAAATT23790.13899965819756183No Hit
GGTATCAACGCAGAGTACTTTTTTT23340.13637040867301775No Hit
GTCCTAAAGTGTGTATTTCTCATTT22440.13111190962392968No Hit
TATCAACGCAGAGTACTTTTTTTTT22150.12941750437477906No Hit
CTGTAGGACGTGGAATATGGCAAGA20920.12223088900769202No Hit
CTTTAGGACGTGAAATATGGCGAGG18650.10896778585054762No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATTT550.00308580212.08191312
TAGGACC5300.012.0033214
TAAGACC1353.7471182E-1011.9568744
TTAAGAC1657.2759576E-1211.5103063
CACGACC600.005898353611.0776934
GTATAAG1853.6379788E-1210.9253761
AAGTCGT1053.4862114E-610.8519367
TGGACAG2550.010.798745
GTCCTAA7950.010.7748611
ATTGCAC1158.86439E-710.7346113
TAAGACT2300.010.7336654
TGGACCG803.7878216E-410.6823745
GTGTAGA3350.010.6303051
CTAATAC1752.382876E-1010.3099453
TAGGACA4700.010.3032644
TGGCGAG13500.010.26552618
TTAGGAC7700.010.2359513
CTTACAC1951.4551915E-1110.2264643
GGCGAGG6250.010.17702219
GTATTGG1907.8216544E-1110.1313011
Produced by FastQC (version 0.11.5)