FastQCFastQC Report
Thu 26 May 2016
SRR1512777_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512777_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1711515
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT48170.2814465546606369No Hit
GGTATCAACGCAGAGTACTTTTTTT29360.17154392453469586No Hit
TATCAACGCAGAGTACTTTTTTTTT28050.16388988702991208No Hit
GTCCTACAGTGGACATTTCTAAATT25040.14630312909907306No Hit
GTCCTAAAGTGTGTATTTCTCATTT24640.14396601841058945No Hit
CTGTAGGACGTGGAATATGGCAAGA23650.13818166945659255No Hit
CTTTAGGACGTGAAATATGGCGAGG22630.13222203720095937No Hit
GTACATGGGAAGCAGTGGTATCAAC17510.10230702038836936No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC4650.012.2665394
TTAGACC907.4044947E-611.6191384
TAATACC1354.6766218E-911.2670424
TCTATAC1354.6820787E-911.2663833
ACTCCGT700.001493124810.8557576
ACGAAAT1053.4752302E-610.85480512
AATGCCG1252.2547465E-710.63491118
ATGCCGG1252.2547465E-710.63491119
CGAAATC1106.069218E-610.36019313
GGCGAGG5800.010.31403119
TATACTG3350.010.216615
AATACGT856.548384E-410.0663665
CTAGCAC1701.540684E-910.0651883
GGTATCA13050.09.9176711
ATAGAAC1752.5447662E-99.7776113
ATAGGAC2054.0017767E-119.7378653
TAGAACT2550.09.692974
GTCCTAC12300.09.67132951
GTACTAG900.00109600249.5165881
TTATACC1401.0483527E-69.5065674
Produced by FastQC (version 0.11.5)