FastQCFastQC Report
Thu 26 May 2016
SRR1512776_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512776_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1838175
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT42590.23169719966814908No Hit
GTACATGGGAAGCAGTGGTATCAAC31100.1691895494172209No Hit
GTCCTACAGTGGACATTTCTAAATT26330.1432398982686632No Hit
GGTATCAACGCAGAGTACTTTTTTT24560.1336107824336638No Hit
GTCCTAAAGTGTGTATTTCTCATTT22780.12392726481428591No Hit
TATCAACGCAGAGTACTTTTTTTTT22680.12338324697050063No Hit
CTGTAGGACGTGGAATATGGCAAGA21960.11946631849524664No Hit
CCCATGTACTCTGCGTTGATACCAC21430.11658302392318469No Hit
CTTTAGGACGTGAAATATGGCGAGG18800.10227535463163191No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCAT400.005279823714.24989814
TAGGACC6900.013.4834314
GTCTTAC1405.493348E-1011.6840161
TCCAACG1303.3092874E-810.95186818
GCCGGTT700.001499218610.85053211
TAATACT1752.0008883E-1110.8496414
TGTAGGA17350.010.7801732
GACGTGA8600.010.5981937
AGGACGT15600.010.5888325
AGGACCT11750.010.584175
GTAGGAC17700.010.3524483
GGCGAGG7000.010.30856919
TTAGGAC9700.010.2772433
GGACGTG16150.010.2287826
TGGCGAG15150.010.21210418
GTAGAAC5150.010.1394533
TACGCTG750.002663178610.1263315
TACACCG750.002663178610.1263315
ACCGATC750.002722970410.0992618
GTTAGAC856.6311547E-410.0526983
Produced by FastQC (version 0.11.5)