FastQCFastQC Report
Thu 26 May 2016
SRR1512776_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512776_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1838175
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT49240.26787438627987No Hit
GTACATGGGAAGCAGTGGTATCAAC34770.18915500428414053No Hit
GGTATCAACGCAGAGTACTTTTTTT30620.16657826376705157No Hit
TATCAACGCAGAGTACTTTTTTTTT28650.15586111224448163No Hit
GTCCTACAGTGGACATTTCTAAATT28300.15395704979123315No Hit
CTGTAGGACGTGGAATATGGCAAGA26020.14155344295292888No Hit
GTCCTAAAGTGTGTATTTCTCATTT24810.13497082704312702No Hit
CCCATGTACTCTGCGTTGATACCAC24280.13208753247106506No Hit
CTTTAGGACGTGAAATATGGCGAGG22590.12289363091109388No Hit
GTCCTACAGTGTGCATTTCTCATTT18590.10113291715968284No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC5650.012.2832584
CGAAATC1052.7306305E-711.75860313
CGTGCGC752.0775215E-411.3980410
ACGAAAT1104.9822665E-711.22503812
GTCCTAC15800.011.1378741
GCGTGCG951.3630517E-510.998119
TCCTACA16550.010.9151642
GCGCCAC700.00149512410.85409513
GGCGAGG6300.010.55029619
TGGCGAG15500.010.53668318
GTAGGAC15150.010.4795583
TACACCG1002.3791497E-510.457885
AAGGCGT1002.4035806E-510.4482036
TATGGCG15800.010.27680216
CCTACAG16600.010.1941513
TAAGCTG1502.4161636E-810.1409755
TAATACC1502.417255E-810.1406984
AACCGTG750.00265174310.1315917
GTCTTAC1606.040864E-910.1068681
AATGCCG1606.2227627E-910.0887218
Produced by FastQC (version 0.11.5)