FastQCFastQC Report
Thu 26 May 2016
SRR1512775_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512775_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1487053
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT35100.23603731676006168No Hit
GGTATCAACGCAGAGTACTTTTTTT21530.1447830036992629No Hit
GTACATGGGAAGCAGTGGTATCAAC21330.1434380617234221No Hit
GTCCTAAAGTGTGTATTTCTCATTT21150.14222761394516537No Hit
GTCCTACAGTGGACATTTCTAAATT21130.1420931197475813No Hit
CTGTAGGACGTGGAATATGGCAAGA19310.12985414776742996No Hit
TATCAACGCAGAGTACTTTTTTTTT18610.12514685085198712No Hit
CTTTAGGACGTGAAATATGGCGAGG17170.1154632686259333No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCCGC400.00530806614.23818810
TTGGACT1900.012.99317554
TACACGG604.1099818E-412.6600175
TAGGACC3950.012.4997644
GTACTAA1103.1956006E-812.24519351
CAGTGCG658.150311E-411.6703879
GTAACAC1001.9414038E-611.3940163
GTCCTAG2200.011.3705371
CTTACAC1701.2732926E-1111.1706043
TACTAGC700.001497874410.8514432
TGTAGGA13650.010.5732012
GTAGGAC12500.010.5584553
CTAGACA1355.7279976E-810.55001454
GGATAGT909.5674965E-510.5496586
ATAAGAC1002.4120924E-510.4445153
GCTTAGG1201.2942401E-610.4229921
GTATACG650.00968773310.3613171
GTCCTAC11650.010.1580291
CATTTCT15450.010.14579414
CCCTATA750.002658998410.1280142
Produced by FastQC (version 0.11.5)