FastQCFastQC Report
Thu 26 May 2016
SRR1512775_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512775_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1487053
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT38470.25869958905297924No Hit
GGTATCAACGCAGAGTACTTTTTTT24830.1669745463006362No Hit
CTGTAGGACGTGGAATATGGCAAGA23770.15984635382867995No Hit
GTCCTACAGTGGACATTTCTAAATT22950.15433209172773263No Hit
TATCAACGCAGAGTACTTTTTTTTT22940.1542648446289406No Hit
GTACATGGGAAGCAGTGGTATCAAC22640.15224743166517937No Hit
GTCCTAAAGTGTGTATTTCTCATTT21870.14706940505819227No Hit
CTTTAGGACGTGAAATATGGCGAGG20820.14000845968502804No Hit
CCCATGTACTCTGCGTTGATACCAC16720.11243714918029149No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACCGT350.002172939516.2825726
GCACCGT551.9632728E-413.8155156
TAGGACC3950.012.7546734
ATGACCG657.9772825E-411.7002855
GACGTGG6900.011.2876767
TGTAGGA14250.011.2754922
ACCGTGC600.005883900411.0811948
GTAGGAC13750.010.9230763
ACCGGCC700.001493739110.8550488
GGACGTG12950.010.7817026
CTGTAGG14700.010.6180271
GTCCTAC12950.010.4360431
TGGACTG3100.010.4264635
AGGACGT13700.010.3391665
GTCTAGA1303.7442624E-710.2494831
GTATTAT1605.984475E-910.1122121
GTCCTAA6600.010.0941871
ATCTAGG856.482734E-410.0772231
ATTACTC856.5554294E-410.0650083
CTAGTAC1054.076252E-59.9585013
Produced by FastQC (version 0.11.5)