FastQCFastQC Report
Thu 26 May 2016
SRR1512774_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512774_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1750529
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT41410.23655706360762946No Hit
GGTATCAACGCAGAGTACTTTTTTT24880.1421284651668153No Hit
TATCAACGCAGAGTACTTTTTTTTT22450.12824694706571557No Hit
GTACATGGGAAGCAGTGGTATCAAC17520.10008403174126221No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGCG400.005273240614.25252216
GCCGCGT655.6335288E-513.1083158
GTCCTAC7800.011.8417871
TAGGACC3200.011.5711944
TTACCCT1406.87578E-1011.5288084
CGCAGGA600.00589860411.0776522
TCCTACA8750.011.0686092
GCTAAAC951.3680952E-510.9943623
TACCGTG951.3694025E-510.9934157
TCTTATA2250.010.972152
TATTAGG1053.4877448E-610.8515782
GTCTTAC1907.2759576E-1210.6342361
GTGTAGA3450.010.5975421
TTATACC909.5736075E-510.5492364
GTTATAT1502.0067091E-810.2628821
GTCTAGT1355.413658E-79.9778021
CCTACAG9250.09.9570563
CTACAGT9500.09.7941324
ACCGTGC1451.7005368E-79.7935698
TGGCGAG7050.09.69576218
Produced by FastQC (version 0.11.5)