FastQCFastQC Report
Thu 26 May 2016
SRR1512774_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512774_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1750529
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT47310.270261161054744No Hit
GGTATCAACGCAGAGTACTTTTTTT30430.17383316700266033No Hit
TATCAACGCAGAGTACTTTTTTTTT28340.1618939189239367No Hit
GTACATGGGAAGCAGTGGTATCAAC20260.11573644309805779No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATAGCG350.002159790816.2976065
GTACCGT1001.0091753E-813.2982996
ACCGTCC500.00150144813.297928
GCACCGT907.4725776E-611.6096276
TAGGACC3150.011.4683574
AAGGCGT752.0765643E-411.3985416
AACACCG803.7458332E-410.6953045
GTATTAC1355.549009E-810.5732431
GTCCTAC7700.010.3810021
ATTATAC1201.5099886E-610.2988863
TAGGACG6850.010.2699744
TGTACCG1303.8027792E-710.2382385
TTAGGAC4900.010.0887043
GTCCTAA4250.010.0756791
TATTATA2002.5465852E-119.9822842
GTAGGAC7150.09.9720253
GGTATCA13850.09.9625141
GGACGTG7050.09.8356226
GTAGTAC1651.0100848E-89.7947453
CTATTAT1752.4992914E-99.7878021
Produced by FastQC (version 0.11.5)