FastQCFastQC Report
Thu 26 May 2016
SRR1512773_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512773_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1514258
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT38010.25101402799258776No Hit
GTCCTACAGTGGACATTTCTAAATT26340.1739465797770261No Hit
CTGTAGGACGTGGAATATGGCAAGA25100.16575775066071965No Hit
GTCCTAAAGTGTGTATTTCTCATTT24620.1625878813253752No Hit
GGTATCAACGCAGAGTACTTTTTTT24030.158691583600681No Hit
CTTTAGGACGTGAAATATGGCGAGG21890.1445592494806037No Hit
TATCAACGCAGAGTACTTTTTTTTT21680.1431724316463905No Hit
GTACATGGGAAGCAGTGGTATCAAC20810.13742704347607873No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCGC400.00541280414.1963338
GGCCTAT751.3003295E-512.8257891
CTAATAC1201.007902E-811.8695813
ATTACAC1459.822543E-1111.7877223
TAGGACC3400.011.4498934
GTTTAGG1951.8189894E-1210.852591
AAGTTAG700.001496629310.8525497
ACCTAAG803.3594886E-410.821761
TTAGTAC1002.4103445E-510.4452313
GTCCTAC11700.010.4415071
GTCCTAA7200.010.2873511
GTAGGCC1201.5320493E-610.286973
TTAGGAC9100.010.2261013
GTATTAG1605.0240487E-910.2205511
TCCTATG1502.460365E-810.1287092
CCGGTTT856.632551E-410.05222912
GTGTAGG2700.09.9756141
TCCAACG1151.0377773E-59.903584518
GGACGTG15750.09.8875486
AGGACGT15750.09.887225
Produced by FastQC (version 0.11.5)