FastQCFastQC Report
Thu 26 May 2016
SRR1512773_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512773_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1514258
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT41280.27260876283962177No Hit
CTGTAGGACGTGGAATATGGCAAGA29250.19316391262255175No Hit
GTCCTACAGTGGACATTTCTAAATT28550.1885411865085078No Hit
GTCCTAAAGTGTGTATTTCTCATTT27490.1815410583929555No Hit
CTTTAGGACGTGAAATATGGCGAGG26060.1720974893314085No Hit
GGTATCAACGCAGAGTACTTTTTTT25870.170842749386168No Hit
TATCAACGCAGAGTACTTTTTTTTT24860.16417281599304742No Hit
GTACATGGGAAGCAGTGGTATCAAC23060.15228580598550578No Hit
CCCATGTACTCTGCGTTGATACCAC15740.10394529862150308No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC400.005298538614.24207515
CCAACGT551.9682669E-413.81140919
CGAAATC500.001503568513.29523813
TAGGACC3600.012.1481844
TCCAACG1104.9988375E-711.2217718
ACGAAAT600.00588543411.08082812
GGCGAGG7000.010.98746919
CCCCTAA700.001470867510.8751661
TCAGTAC1105.988757E-610.37157253
GTCCTAC14250.010.3504871
TATACTG2500.010.2681965
TGTAGGA13550.010.2450172
AGGACGT17050.010.1488645
ATATACT1502.4141627E-810.1410934
GACGTGA9200.010.0143727
GGACGTG16400.09.9618216
TAACCTG1252.542749E-69.8878935
GTAAGAC1252.543824E-69.8875663
GTAGGAC13850.09.8848193
TAGGACG18000.09.8241834
Produced by FastQC (version 0.11.5)