FastQCFastQC Report
Thu 26 May 2016
SRR1512772_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512772_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1917242
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT36090.18823914769236225No Hit
GGTATCAACGCAGAGTACTTTTTTT23670.1234585931249159No Hit
GTACATGGGAAGCAGTGGTATCAAC22190.11573917116357768No Hit
GTCCTACAGTGGACATTTCTAAATT20660.10775895791976181No Hit
TATCAACGCAGAGTACTTTTTTTTT19650.10249097401371346No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCTA250.006048903318.9911065
CGTTTAT500.001365194213.4668991
TCCAACG1701.8189894E-1211.72672518
GGACCGT658.056463E-411.6868346
TAGGACC6500.011.5407494
TTAGGGT1604.5474735E-1111.2759694
GTCTTAC1656.366463E-1111.0766691
GTCTAGC1157.509516E-710.8738921
GGCGAGG5200.010.77210519
CCAACGA1158.889929E-710.73241319
GTGTAGG3450.010.5950751
GTCCTAC12150.010.4505031
CTAGGAC2850.010.3287683
AAGTCGT1303.8652252E-710.2262497
GTATAAA3200.010.2204131
TGTAGGA14800.010.137412
TTATACT2250.010.1285894
GCCGTGT750.00273211910.0952188
GTAGGAC13550.010.02143
TCCTACA14150.09.9991132
Produced by FastQC (version 0.11.5)