##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512772_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1917242 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.394887030432255 31.0 25.0 33.0 10.0 34.0 2 27.925417865871914 31.0 26.0 34.0 16.0 34.0 3 29.02840277857464 31.0 28.0 34.0 16.0 34.0 4 32.84463255029881 35.0 33.0 37.0 25.0 37.0 5 33.198763119105465 37.0 35.0 37.0 27.0 37.0 6 33.47859685944706 37.0 35.0 37.0 28.0 37.0 7 33.46938153868943 37.0 35.0 37.0 28.0 37.0 8 33.502649639429976 37.0 35.0 37.0 28.0 37.0 9 35.02128526289326 39.0 35.0 39.0 28.0 39.0 10 34.926725473362254 39.0 35.0 39.0 28.0 39.0 11 34.980915293948286 39.0 35.0 39.0 28.0 39.0 12 34.87481705491534 39.0 35.0 39.0 27.0 39.0 13 34.86392171671599 39.0 35.0 39.0 27.0 39.0 14 35.98662870936481 40.0 37.0 41.0 27.0 41.0 15 35.892214441369426 40.0 37.0 41.0 27.0 41.0 16 35.78897395320987 40.0 36.0 41.0 27.0 41.0 17 35.73672076868752 40.0 36.0 41.0 27.0 41.0 18 35.63749020728734 40.0 36.0 41.0 26.0 41.0 19 35.66433241082764 40.0 36.0 41.0 26.0 41.0 20 35.568944348183486 40.0 36.0 41.0 26.0 41.0 21 35.46995736584114 40.0 36.0 41.0 25.0 41.0 22 35.33689904560822 40.0 36.0 41.0 25.0 41.0 23 35.18688198985835 40.0 36.0 41.0 24.0 41.0 24 35.0495169623866 40.0 36.0 41.0 22.0 41.0 25 34.9259420563497 40.0 36.0 41.0 19.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 3.519921092608733 1101 2 2.096036133509884 1101 3 1.0581312012713688 1101 4 0.8571980687346681 1101 5 0.5310014205651257 1101 6 0.3932045145601677 1101 7 0.10161494337049248 1101 8 0.14681283937910905 1101 9 -0.13321586736398672 1101 10 -3.307075111891322E-4 1101 11 0.12473087897943458 1101 12 0.18020412465700275 1101 13 0.20558856394511338 1101 14 -0.24322382207594728 1101 15 -0.0970990883328966 1101 16 -0.18151665159926011 1101 17 -0.5620715525021893 1101 18 -0.4882291482043968 1101 19 -0.5049972397237354 1101 20 -0.4695800329448758 1101 21 -0.5417622822714705 1101 22 -0.5634185179354674 1101 23 -0.5147398927100397 1101 24 -0.5957526472709489 1101 25 -0.6930302306053235 1102 1 3.946464018088804 1102 2 2.478182460732185 1102 3 1.36057333348802 1102 4 1.3992355560191925 1102 5 0.9249136522096038 1102 6 0.7622643521973771 1102 7 0.5798560790281186 1102 8 0.461952204068929 1102 9 0.6432175035265786 1102 10 0.41238142621079277 1102 11 0.3426403554134083 1102 12 0.3981622582299664 1102 13 0.3775018386769986 1102 14 0.10439418289012536 1102 15 0.1531544609773121 1102 16 -0.0350191293120119 1102 17 0.11535746509497358 1102 18 0.11914575353099366 1102 19 0.1667563787708417 1102 20 0.20999217957125893 1102 21 0.20071220694261172 1102 22 0.30300775205387254 1102 23 0.12357790835221039 1102 24 0.3323883611178431 1102 25 0.30790185771346756 1103 1 2.5655098554754154 1103 2 1.2725960032562753 1103 3 0.31651139491110314 1103 4 -0.1433729583286123 1103 5 -0.42048038405021515 1103 6 -0.5176430891428012 1103 7 -0.5752113349128578 1103 8 -0.5885447742796828 1103 9 -1.0552940237744508 1103 10 -1.1057679889978118 1103 11 -1.0322612306577668 1103 12 -1.0660719934628133 1103 13 -1.2346478285224265 1103 14 -1.4152642991913424 1103 15 -1.3994876372236362 1103 16 -1.5901693036909421 1103 17 -1.5398471367435391 1103 18 -1.5993862528212262 1103 19 -1.6281979207106545 1103 20 -1.5142093005230635 1103 21 -1.55977723086842 1103 22 -1.6308580877758985 1103 23 -1.726686458162689 1103 24 -1.6164507862321855 1103 25 -1.7476896203917889 1104 1 -6.534359339582728 1104 2 -7.918829090121818 1104 3 -7.5752976798925715 1104 4 -5.97196904732483 1104 5 -3.972187885821228 1104 6 -2.6945543011445174 1104 7 -2.177294152063954 1104 8 -1.7419174944990239 1104 9 -1.7530191577491934 1104 10 -1.761566911151128 1104 11 -1.6890994966576685 1104 12 -1.8817809748593177 1104 13 -1.8826217187160665 1104 14 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0.03264669782274865 2314 21 -0.12505326119980253 2314 22 -0.42742407816621153 2314 23 -0.28318836059150954 2314 24 -0.3018450159792394 2314 25 -0.04211107058343799 2315 1 -0.23595823790657633 2315 2 0.9450721488077214 2315 3 0.6639107205124724 2315 4 0.5519375075819184 2315 5 0.3557770154599993 2315 6 0.186549017070206 2315 7 0.1624218785514273 2315 8 0.0787126436915031 2315 9 -0.5633948920133562 2315 10 -0.3244751985190888 2315 11 -0.08408883567685876 2315 12 -0.08201272990306308 2315 13 -0.07063667809155305 2315 14 -0.056987637878364694 2315 15 -0.028869493646880073 2315 16 -0.07821009099437504 2315 17 -0.09907663916924747 2315 18 -0.054520028072182924 2315 19 -0.16165780723293466 2315 20 -0.07604816673890014 2315 21 -0.044307885959412374 2315 22 -0.5978651366866714 2315 23 -0.15150858027123348 2315 24 0.10685288858468311 2315 25 0.2731171752852859 2316 1 0.7724281774627855 2316 2 0.9727390892035785 2316 3 0.5036548369312435 2316 4 0.334739708370293 2316 5 0.13972110261855164 2316 6 -0.11703368511796697 2316 7 -0.15806335291433982 2316 8 -0.2523011575669116 2316 9 -0.21506065973008504 2316 10 -0.3343480386091642 2316 11 -0.2832862088983106 2316 12 -0.28651610724450194 2316 13 -0.2982208611702646 2316 14 -0.3038845577651088 2316 15 -0.29963080278476895 2316 16 -0.22014016702127037 2316 17 -0.07786383295688637 2316 18 -0.08522126152799103 2316 19 0.0937227309762676 2316 20 -0.24384373573164453 2316 21 -0.07793585066722386 2316 22 -0.044926208947345 2316 23 0.03981833369392973 2316 24 -0.15350515033367884 2316 25 -0.16973763429555078 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 85795.0 3 6.0 4 738.0 5 3056.0 6 3770.0 7 3497.0 8 3107.0 9 3066.0 10 2908.0 11 2992.0 12 2961.0 13 3085.0 14 3009.0 15 2944.0 16 2994.0 17 3379.0 18 3777.0 19 4587.0 20 5489.0 21 6602.0 22 8016.0 23 9267.0 24 10666.0 25 12197.0 26 13813.0 27 15775.0 28 18427.0 29 22270.0 30 27821.0 31 36264.0 32 47719.0 33 65327.0 34 94094.0 35 137489.0 36 234108.0 37 329820.0 38 686407.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.7302808354 20.65520299362459 11.70325287604596 23.911263294929444 2 14.773127328138216 20.172184799967777 41.17348889738642 23.88119897450759 3 18.99338694627966 24.426248028217927 29.485202743130667 27.095162282371742 4 12.308543673984326 16.764677729895354 38.32421742398367 32.602561172136646 5 13.344983803196678 37.604777076227236 35.63124118303355 13.418997937542532 6 31.96625645823479 36.722235143773304 18.179019524318505 13.1324888736734 7 28.08805144805053 31.37717008476521 22.1748160660936 18.359962401090662 8 25.222755634376465 36.428977002395655 20.74092586454073 17.607341498687152 9 26.505530969981905 15.433345565135392 20.123666655166684 37.937456809716025 10 14.968000409728543 28.942931712257682 34.49182685624277 21.597241021771012 11 34.432834244617254 22.49987713613212 24.288461357483666 18.77882726176696 12 23.228788596488474 24.76626963089371 31.61181675497236 20.393125017645453 13 28.91685394668454 21.78344608479314 25.757216075998212 23.542483892524103 14 21.168315723577653 22.014609045642416 27.68684532161709 29.130229909162843 15 23.364979041114623 30.103151979555065 23.50025173714346 23.03161724218685 16 22.48102301361099 28.0998890878723 27.387639197342306 22.031448701174412 17 22.115793519577444 28.178034646921162 27.627975314973202 22.07819651852819 18 21.867078414804528 27.64314278304373 29.066988898277437 21.42278990387431 19 23.547598339096872 26.985494277578226 27.51001476079566 21.95689262252924 20 23.550213162058043 27.248471646075203 27.9671128862566 21.234202305610157 21 23.29688211256625 26.621338091176888 27.223201812445392 22.85857798381147 22 23.059532780708363 27.701687180775348 27.245562254040024 21.99321778447626 23 22.687618948834125 27.312250086305845 27.48700959553336 22.51312136932667 24 22.95085484734476 27.007827574603215 28.043148216279075 21.998169361772945 25 23.030764914513988 26.981012181180937 27.601324897094354 22.386898007210725 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1585.0 1 1585.0 2 1382.5 3 1180.0 4 1180.0 5 1180.0 6 1908.0 7 2636.0 8 2636.0 9 2636.0 10 3407.0 11 4178.0 12 4178.0 13 4178.0 14 6513.5 15 8849.0 16 8849.0 17 8849.0 18 15420.5 19 21992.0 20 21992.0 21 21992.0 22 34903.0 23 47814.0 24 47814.0 25 47814.0 26 68168.0 27 88522.0 28 88522.0 29 88522.0 30 113601.0 31 138680.0 32 138680.0 33 138680.0 34 158555.5 35 178431.0 36 178431.0 37 178431.0 38 193746.5 39 209062.0 40 209062.0 41 209062.0 42 222181.0 43 235300.0 44 235300.0 45 235300.0 46 244218.0 47 253136.0 48 253136.0 49 253136.0 50 244540.0 51 235944.0 52 235944.0 53 235944.0 54 211201.0 55 186458.0 56 186458.0 57 186458.0 58 160221.0 59 133984.0 60 133984.0 61 133984.0 62 109510.5 63 85037.0 64 85037.0 65 85037.0 66 66171.0 67 47305.0 68 47305.0 69 47305.0 70 34748.5 71 22192.0 72 22192.0 73 22192.0 74 15642.0 75 9092.0 76 9092.0 77 9092.0 78 6348.5 79 3605.0 80 3605.0 81 3605.0 82 2488.5 83 1372.0 84 1372.0 85 1372.0 86 949.0 87 526.0 88 526.0 89 526.0 90 360.0 91 194.0 92 194.0 93 194.0 94 148.5 95 103.0 96 103.0 97 103.0 98 84.0 99 65.0 100 65.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.7197098749140691 2 0.2907822799625712 3 0.27466537870545293 4 0.2807678947154298 5 0.28400170661815255 6 0.20795496864767202 7 0.45252503335520505 8 0.24034524593139517 9 0.21885604425523744 10 0.19715820955309762 11 0.2376330165936277 12 0.23825891567157406 13 0.24910783302264397 14 0.2680412801305208 15 0.4436059714944696 16 0.5386383148293226 17 0.5307102598420022 18 0.2475430853277781 19 0.2120754709108188 20 0.2038866246410208 21 0.31357543805111715 22 0.33183082782455214 23 0.4346347513772388 24 0.33553406403573466 25 0.3522768643707993 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1917242.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.71017229573098 #Duplication Level Percentage of deduplicated Percentage of total 1 88.32808332311645 69.52318656914179 2 8.334815265654228 13.12069491225466 3 1.7654578124858842 4.1687846580482475 4 0.6056837713899321 1.9069389601131879 5 0.29629372505045437 1.1660665074432603 6 0.16312502040109333 0.7703759076908814 7 0.1054502072071743 0.5810002784528057 8 0.0757171200229501 0.47677660501943564 9 0.05302846373116003 0.37564915651717745 >10 0.24536183078518992 3.3655700540710107 >50 0.013707344375688723 0.7648338929052725 >100 0.010808813895748929 1.6899867298812643 >500 0.0015337281981889928 0.8171694987486726 >1k 9.335736858541556E-4 1.272966269712334 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3609 0.18823914769236225 No Hit GGTATCAACGCAGAGTACTTTTTTT 2367 0.1234585931249159 No Hit GTACATGGGAAGCAGTGGTATCAAC 2219 0.11573917116357768 No Hit GTCCTACAGTGGACATTTCTAAATT 2066 0.10775895791976181 No Hit TATCAACGCAGAGTACTTTTTTTTT 1965 0.10249097401371346 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 5.2158256495528475E-5 0.0 0.0 0.0 8 0.0 5.2158256495528475E-5 0.0 0.0 5.2158256495528475E-5 9 0.0 5.2158256495528475E-5 0.0 0.0 5.2158256495528475E-5 10 0.0 5.2158256495528475E-5 0.0 0.0 5.2158256495528475E-5 11 0.0 5.2158256495528475E-5 0.0 0.0 5.2158256495528475E-5 12 0.0 5.2158256495528475E-5 0.0 0.0 5.2158256495528475E-5 13 0.0 5.2158256495528475E-5 0.0 0.0 5.2158256495528475E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGCTA 25 0.0060489033 18.991106 5 CGTTTAT 50 0.0013651942 13.466899 1 TCCAACG 170 1.8189894E-12 11.726725 18 GGACCGT 65 8.056463E-4 11.686834 6 TAGGACC 650 0.0 11.540749 4 TTAGGGT 160 4.5474735E-11 11.275969 4 GTCTTAC 165 6.366463E-11 11.076669 1 GTCTAGC 115 7.509516E-7 10.873892 1 GGCGAGG 520 0.0 10.772105 19 CCAACGA 115 8.889929E-7 10.732413 19 GTGTAGG 345 0.0 10.595075 1 GTCCTAC 1215 0.0 10.450503 1 CTAGGAC 285 0.0 10.328768 3 AAGTCGT 130 3.8652252E-7 10.226249 7 GTATAAA 320 0.0 10.220413 1 TGTAGGA 1480 0.0 10.13741 2 TTATACT 225 0.0 10.128589 4 GCCGTGT 75 0.002732119 10.095218 8 GTAGGAC 1355 0.0 10.0214 3 TCCTACA 1415 0.0 9.999113 2 >>END_MODULE