FastQCFastQC Report
Thu 26 May 2016
SRR1512772_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512772_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1917242
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT44950.2344513629474005No Hit
GGTATCAACGCAGAGTACTTTTTTT27560.14374815490167647No Hit
TATCAACGCAGAGTACTTTTTTTTT26140.13634168247931144No Hit
GTACATGGGAAGCAGTGGTATCAAC25450.13274276278111996No Hit
GTCCTACAGTGGACATTTCTAAATT22320.11641722849801955No Hit
CTGTAGGACGTGGAATATGGCAAGA20100.10483809555601224No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA1205.2750693E-1113.45055519
GCCGGTT1600.013.06074211
TCCAACG1850.012.31720218
CGGTTTC1900.011.49665213
TAGGACC6900.011.4349514
AGGACGT12850.010.8753225
GGACCGA803.7753204E-410.6863416
TGTAGGA14650.010.6433792
TAGGACG13300.010.5068114
GGACGTG12500.010.4868626
GTAGGAC14800.010.4051243
GTAACAC1659.1858965E-1010.3700893
CCGGTTT1856.002665E-1110.268352512
CTCGAAC1303.872501E-710.22485718
GGTCTAC2151.8189894E-1210.1816151
ACCGTGC856.602603E-410.0574698
AGGACCT11200.010.0159455
GTCCTAC10950.09.9087311
TCGAACT1151.0336162E-59.90731419
GGACCTG10550.09.9041396
Produced by FastQC (version 0.11.5)