FastQCFastQC Report
Thu 26 May 2016
SRR1512771_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512771_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1479288
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT36940.24971472762572267No Hit
GTCCTACAGTGGACATTTCTAAATT30700.20753227228234122No Hit
GTCCTAAAGTGTGTATTTCTCATTT29770.2012454640340488No Hit
CTGTAGGACGTGGAATATGGCAAGA28520.19279545294763428No Hit
CTTTAGGACGTGAAATATGGCGAGG25210.17041982359080854No Hit
GGTATCAACGCAGAGTACTTTTTTT22300.1507481977816355No Hit
TATCAACGCAGAGTACTTTTTTTTT20320.13736338022075484No Hit
GTACATGGGAAGCAGTGGTATCAAC16940.11451455024308992No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC4250.012.7329214
GCGCAGA853.4113255E-612.4543151
GTCTTAA1251.1496013E-912.318451
GGACCGG550.003085597212.0818416
GTGTAGG2950.012.0705151
TTAATCC1950.011.1978674
GTATTAT1552.4920155E-1011.1760131
AGGACGT17450.011.0436635
GGACGTG16800.011.0185166
GTAGGAC15200.010.8694313
GGCGAGG9350.010.8675719
GACGTGG7950.010.8664277
TGTAGGA15800.010.6970512
GACGTGA9700.010.6676147
CACCTTT4900.010.66397314
GAATTAT1907.2759576E-1210.6368191
GGGTAAT1002.078666E-510.5861671
TTAGGAC11850.010.5768593
GTAGAAC4050.010.5501513
TAGGACG18950.010.5208834
Produced by FastQC (version 0.11.5)