FastQCFastQC Report
Thu 26 May 2016
SRR1512771_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512771_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1479288
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT42380.28648917587379874No Hit
GTCCTACAGTGGACATTTCTAAATT33440.2260546965837619No Hit
CTGTAGGACGTGGAATATGGCAAGA32720.22118749019798714No Hit
GTCCTAAAGTGTGTATTTCTCATTT31960.2160498834574471No Hit
CTTTAGGACGTGAAATATGGCGAGG28690.19394465445538664No Hit
GGTATCAACGCAGAGTACTTTTTTT26240.17738263272601412No Hit
TATCAACGCAGAGTACTTTTTTTTT24530.16582301755979906No Hit
GTACATGGGAAGCAGTGGTATCAAC19670.1329693744558193No Hit
CTGAAGGACCTGGAATATGGCGAGA16690.112824548025807No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGACC250.006035849518.997878
TAGGACC3950.013.237114
ACCTTTT5250.011.03126115
AGGACGT17600.010.96585
GAATAGG1053.3905762E-610.8768941
GGCGAGG8600.010.81924419
CCACCTT5200.010.77508613
TATACTG1951.8189894E-1210.726185
TGCTATA1252.213419E-710.6488912
TCCAACG909.570082E-510.549376518
CACCTTT5950.010.53164614
GGACGTG17650.010.4942186
TGTAGGA16150.010.3615932
GACGTGA9750.010.3270477
GTAGGAC16350.010.2912563
TGGACTG2151.8189894E-1210.1705945
AGGGACG750.00263063410.1411155
AATGCCG750.002660332510.12740118
GTCCTAG2450.010.0999731
TTAGTAC856.5522897E-410.0655263
Produced by FastQC (version 0.11.5)