FastQCFastQC Report
Thu 26 May 2016
SRR1512770_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512770_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1872395
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT42060.22463208884877392No Hit
GTCCTACAGTGGACATTTCTAAATT29070.15525570192187013No Hit
GGTATCAACGCAGAGTACTTTTTTT25090.13399950330993193No Hit
CTGTAGGACGTGGAATATGGCAAGA23850.12737696906902657No Hit
GTCCTAAAGTGTGTATTTCTCATTT23710.12662926359021467No Hit
TATCAACGCAGAGTACTTTTTTTTT22720.12134191770433055No Hit
CTTTAGGACGTGAAATATGGCGAGG19870.10612077045708838No Hit
GTCCTACAGTGTGCATTTCTCATTT19750.10547988004667819No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACCG707.3066403E-613.5638095
CCAACGA1205.2750693E-1113.44969519
TCCAACG1600.012.45940818
GCGTAGA657.2140276E-411.8416181
TAGGACC6400.011.7202954
GGTATCA9250.011.5455771
TCAATAC1658.185452E-1110.9344283
GTACCGT700.00149898110.8507576
GGCGAGG7250.010.73793119
GTATAAG1907.2759576E-1210.6340861
GTCCTAA8950.010.535071
GTCCTAT2750.010.4959791
TGACGAA1002.4197125E-510.4418910
CTAGGAC2300.010.32143
TTAGGAC10250.010.2831353
GTCCTAC16750.010.2818821
AGGACGT15450.010.201395
TGTAGGA16700.010.1780132
TGGCGAG15700.010.15798918
GTAGGAC15650.010.1327793
Produced by FastQC (version 0.11.5)