FastQCFastQC Report
Thu 26 May 2016
SRR1512770_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512770_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1872395
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT45040.24054753404062712No Hit
GTCCTACAGTGGACATTTCTAAATT30330.16198505123117718No Hit
GGTATCAACGCAGAGTACTTTTTTT28460.1519978423356183No Hit
CTGTAGGACGTGGAATATGGCAAGA28040.1497547258991826No Hit
TATCAACGCAGAGTACTTTTTTTTT27680.147832054667952No Hit
GTCCTAAAGTGTGTATTTCTCATTT25730.1374175854987863No Hit
CTTTAGGACGTGAAATATGGCGAGG25460.13597558207536337No Hit
GTCCTACAGTGTGCATTTCTCATTT21580.11525345880543368No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGACC400.00525500414.2600484
ATAGCGG400.005284909614.2478566
GTCGGCC400.005285846514.24747512
TATACCG500.001491801813.3093785
TAGGACC7400.012.7184214
CCAACGA1501.8189894E-1212.66002619
GGTATCA11600.012.2229581
ACTGCGC550.00307292312.0887678
TCCAACG2300.011.55915418
GGCGAGG8350.011.48499319
AGGACCT11250.011.4080385
GCGCCAC752.080758E-411.39615313
GGAGCGC600.00588300411.0816666
GTAGGAC18250.011.0433973
GGACGTG15950.011.0171516
TGTAGGA19350.010.9557482
AGGACGT16200.010.9150985
GTCCTAA7500.010.784641
GACGTGA9250.010.7818747
GGACCTG11650.010.6807976
Produced by FastQC (version 0.11.5)