FastQCFastQC Report
Thu 26 May 2016
SRR1512769_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512769_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1736462
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT40320.23219627034740753No Hit
GGTATCAACGCAGAGTACTTTTTTT23730.13665717994404716No Hit
GTCCTACAGTGGACATTTCTAAATT22830.13147422748093537No Hit
GTCCTAAAGTGTGTATTTCTCATTT20860.12012932042279072No Hit
TATCAACGCAGAGTACTTTTTTTTT19900.11460083779547148No Hit
CTGTAGGACGTGGAATATGGCAAGA19840.11425530763126404No Hit
CTTTAGGACGTGAAATATGGCGAGG18160.10458046303345539No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG2500.012.3208881
TAGGACC4400.011.221594
ACCGATG600.006051308511.0396658
GACTGTC1951.8189894E-1210.71221357
TTAGGAC8250.010.5898043
GTTTAGG2100.010.5424251
AGGACGT14200.010.4313365
GGCGAGG6200.010.41347819
TGTAGGA14850.010.35959152
GGACGTG14400.010.3520966
TAAGACA2053.6379788E-1210.1899614
TAGGACG15400.010.1734194
GGTCTAT951.439724E-410.132311
ATTATAC1502.4590918E-810.1293773
TTTAGGA9550.010.0431982
GACGTGA7200.010.0224117
TCCAACG951.653932E-49.99406918
GTAGGAC14500.09.823753
GTATAAA2550.09.8144331
GGTATCA11350.09.7529061
Produced by FastQC (version 0.11.5)