FastQCFastQC Report
Thu 26 May 2016
SRR1512769_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512769_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1736462
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT45210.2603569787303148No Hit
GGTATCAACGCAGAGTACTTTTTTT28700.16527859521256438No Hit
TATCAACGCAGAGTACTTTTTTTTT26240.15111185848005887No Hit
GTCCTACAGTGGACATTTCTAAATT24240.1395941863398105No Hit
CTGTAGGACGTGGAATATGGCAAGA22510.12963139993849565No Hit
GTCCTAAAGTGTGTATTTCTCATTT22370.12882516288867826No Hit
CTTTAGGACGTGAAATATGGCGAGG20220.11644366533791123No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAA500.001501488313.29786310
TCCTATA2000.011.881992
TAAGACG752.0638783E-411.40605454
TAAGGCT1751.8189894E-1211.4060544
TAGGACC4000.011.4060544
TAATACC1451.20599E-911.1438454
GCCCTAT600.00580165211.1023411
GACCGTG700.001493555510.8553987
GGTATCA12700.010.7901261
ACAACCG803.7487003E-410.6944085
ATTTAGA4450.010.6924641
GTCCTAT1803.45608E-1110.5736571
GTCCTAC12550.010.4641291
GTCTTAT1752.2919266E-1010.3319741
GTAGCAC1502.4241672E-810.1387153
AATGTCC4900.010.0800138
GTATAGA2750.010.0353611
GTACTAG951.6158883E-410.017151
TTTAGAA5200.09.8711922
TCTAAAC1856.4210326E-109.7619383
Produced by FastQC (version 0.11.5)