FastQCFastQC Report
Thu 26 May 2016
SRR1512768_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512768_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1669484
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT32470.19449123202139104No Hit
GGTATCAACGCAGAGTACTTTTTTT19350.11590407575035161No Hit
TATCAACGCAGAGTACTTTTTTTTT17850.10691926367668095No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCGTT551.9705415E-413.8096777
TCTAAAC1501.7826096E-1011.39473
TAAGACC1451.2296368E-911.1310764
GTCCTAA3500.010.9983111
CTAAGAC1408.409188E-910.8520963
GTACTAG908.346341E-510.6928021
GTCTTAG1907.2759576E-1210.6365241
TAGGCAG1803.6379788E-1110.5490585
GTCCTAG2200.010.4983871
CTTAGAC2001.8189894E-1210.4451433
GTCCTAC7100.010.4367771
CCGTCCA1106.1931514E-610.3426069
TAGGACC3800.09.9938454
GATATAC1951.2732926E-109.8702791
TCCTACA8550.09.7732322
TTAGACT1752.5993359E-99.7654144
GATTAGG800.00405898469.6235231
GTATTAG1408.872503E-79.6235221
GGATAAT1105.9339865E-59.6235221
GTCCATA1303.5942921E-69.6235221
Produced by FastQC (version 0.11.5)