FastQCFastQC Report
Thu 26 May 2016
SRR1512768_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512768_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1669484
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT37550.22491979557755568No Hit
GGTATCAACGCAGAGTACTTTTTTT24530.1469316267780943No Hit
TATCAACGCAGAGTACTTTTTTTTT21890.13111835752843393No Hit
GTACATGGGAAGCAGTGGTATCAAC17280.10350503508868608No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGACT551.949846E-413.8268844
TAGGACC2800.011.882484
TAGACAG3150.011.7700195
TAGACTG2400.011.4870855
ACCGTAT600.005881211711.0820058
GTCGAAG700.00146816810.8776821
GTATAAG1752.0008883E-1110.8776821
GTCTTAA2050.010.67869951
TTAGGAC5100.010.6240163
TGTACCG909.448826E-510.5628375
ATTAGGG1002.3828437E-510.4562673
TAGGACT2550.010.43794254
GGTATAT1201.4869765E-610.3111361
GTATTAC1409.4218194E-810.1978271
TTAGGGT1409.59717E-810.1849824
CTAGTAC750.002634608210.1394113
GCACCGA750.002650380610.1321186
GGACCGT750.002650380610.1321186
TATACTG3300.010.0827085
TGGCGAG7550.09.93503818
Produced by FastQC (version 0.11.5)