FastQCFastQC Report
Thu 26 May 2016
SRR1512767_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512767_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1358624
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT40040.294709941823492No Hit
CTGTAGGACGTGGAATATGGCAAGA36990.27226075794333093No Hit
GTCCTAAAGTGTGTATTTCTCATTT35900.26423793485173236No Hit
GTATCAACGCAGAGTACTTTTTTTT34300.25246131379984454No Hit
CTTTAGGACGTGAAATATGGCGAGG30170.2220629107096592No Hit
GTCCTACAGTGTGCATTTCTCATTT20720.15250724262194693No Hit
GGTATCAACGCAGAGTACTTTTTTT20060.1476493864380432No Hit
TATCAACGCAGAGTACTTTTTTTTT17450.12843877334715123No Hit
CTGAAGGACCTGGAATATGGCGAGA15940.11732458722943212No Hit
GTACATGGGAAGCAGTGGTATCAAC15180.11173069222978543No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGAC602.578081E-514.2439433
TAGGACC5300.012.362214
GGCGAGG8550.011.43576719
ACTACAC1104.9930895E-711.2225013
TCCTATA1950.011.2003652
TTATACT1303.2856406E-810.9564744
GTATAAC1501.720764E-910.9063341
CCTTATC1053.482195E-610.8525282
GTCCTAT2050.010.7968011
GACGTGG10700.010.7368347
GGACGTG20750.010.707516
AGGACGT21150.010.6849725
ACCTTTT5450.010.63284115
GTAGGAC19300.010.6276053
TGGCGAG18900.010.4961618
TGTAGGA19950.010.4717382
TAATTCA2100.010.3999544
CACCTTT5300.010.39604414
ATTTCTA12100.010.36345515
TAGGACG22550.010.3588484
Produced by FastQC (version 0.11.5)