FastQCFastQC Report
Thu 26 May 2016
SRR1512767_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512767_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1358624
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT44120.3247403255058059No Hit
CTGTAGGACGTGGAATATGGCAAGA41150.3028799726782392No Hit
GTATCAACGCAGAGTACTTTTTTTT40490.29802211649433547No Hit
GTCCTAAAGTGTGTATTTCTCATTT39940.293973903007749No Hit
CTTTAGGACGTGAAATATGGCGAGG35710.26283946110182066No Hit
GGTATCAACGCAGAGTACTTTTTTT23170.17054019360765008No Hit
TATCAACGCAGAGTACTTTTTTTTT23090.1699513625550557No Hit
GTCCTACAGTGTGCATTTCTCATTT21340.15707068327955345No Hit
CTGAAGGACCTGGAATATGGCGAGA17820.13116211696540028No Hit
GTACATGGGAAGCAGTGGTATCAAC16730.12313929387380172No Hit
CTGTAGGACCTGGAATATGGCGAGA15150.11150988058506253No Hit
ATTTAGAAATGTCCACTGTAGGACG14290.10517994676967285No Hit
GTCCTTCAGTGTGCATTTCTCATTT13690.10076371387521493No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC5550.014.3909054
TATGTCG957.446033E-812.99168816
GATTGAT604.0982635E-412.66416211
AGGACGT19950.011.53469855
GGACGTG19600.011.4849746
TGTAGGA20250.011.4573082
CTAGGAC2100.011.3193773
AAGTCGT1604.5474735E-1111.279027
GGCGAGG9100.011.26793219
CTACACT2200.011.2370534
GTCCTAC19350.011.1155181
TAGAACG600.005837049311.0929894
CCATGCG600.00588366211.0811419
GTAGGAC19850.011.0650473
TGTCGAG951.3688472E-510.99337118
CTGTAGG20450.010.9829991
ATGTCGA1053.4896639E-610.85060117
GACGTGA11000.010.7933217
TAGGACG21900.010.7239474
ACCTTTT4550.010.64193515
Produced by FastQC (version 0.11.5)