FastQCFastQC Report
Thu 26 May 2016
SRR1512766_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512766_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1684927
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT42280.25093075248957375No Hit
GTCCTACAGTGGACATTTCTAAATT36560.21698269420574304No Hit
GTCCTAAAGTGTGTATTTCTCATTT31990.18985985743002515No Hit
CTGTAGGACGTGGAATATGGCAAGA31450.18665497080882434No Hit
CTTTAGGACGTGAAATATGGCGAGG28730.17051183819833143No Hit
GTACATGGGAAGCAGTGGTATCAAC26130.15508090261477203No Hit
GGTATCAACGCAGAGTACTTTTTTT24340.14445729696301382No Hit
TATCAACGCAGAGTACTTTTTTTTT22070.13098490320352157No Hit
CCCATGTACTCTGCGTTGATACCAC18300.10861004660736044No Hit
GTCCTACAGTGTGCATTTCTCATTT18140.1076604505714491No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCCG456.786545E-414.7709225
TAGGACC5750.012.5506964
TTAGGAC13400.011.1260983
GTCCTAC18050.011.0914211
GGCGAGG9150.011.00068819
AGGACCT11400.010.8283085
TCCTACA20000.010.7306612
GGACTGT2750.010.7038036
AGGACGT19900.010.6885075
GGACGTG19300.010.6760726
GACGTGA11150.010.6446477
CCTACAG19650.010.6318333
CTTATAC1701.4006218E-1010.6133563
GTGTAGG2850.010.4693061
TGTAGGA19200.010.4358842
GGACCTG11400.010.3282316
GTAGGAC18700.010.2578853
TAGGACG21900.010.2326944
AGGACCG750.00265796410.1286335
CTACAGT21650.010.0876964
Produced by FastQC (version 0.11.5)