FastQCFastQC Report
Thu 26 May 2016
SRR1512765_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512765_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1783144
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT46440.2604388652851368No Hit
GGTATCAACGCAGAGTACTTTTTTT29240.1639800262906417No Hit
TATCAACGCAGAGTACTTTTTTTTT25410.14250111039826285No Hit
GTACATGGGAAGCAGTGGTATCAAC22460.1259572978963No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG350.0021789716.2760665
TACATCG550.003082387812.0837465
TAGGACC3500.011.6648434
TAGGACT2000.011.3935664
GATCTAA951.1820435E-511.1402671
GTCTTAG2250.010.6901561
GTATTAG1303.227633E-710.3612281
GTGTTAG2150.010.2923831
TCAATAC1502.460547E-810.1290443
GAATAGG1158.828249E-610.0394511
ATCCTAT1553.338755E-89.93151
ATAGGCC1151.0315758E-59.9088473
ATCCTAC1605.4551492E-89.62114051
CCCCTAA909.917354E-49.62114051
GATATAC1701.3531462E-89.621141
TGTAGGA9300.09.5980852
CTCACGA800.00453177379.4959792
TTAGACA2106.730261E-119.4946384
TTAGGAC5700.09.3293833
CTTATAC2252.7284841E-119.2849583
Produced by FastQC (version 0.11.5)