FastQCFastQC Report
Thu 26 May 2016
SRR1512765_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512765_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1783144
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT56010.3141081146559111No Hit
GGTATCAACGCAGAGTACTTTTTTT35720.20032033307461425No Hit
TATCAACGCAGAGTACTTTTTTTTT33210.18624407226785947No Hit
GTACATGGGAAGCAGTGGTATCAAC25200.14132341527100448No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGA604.0951508E-412.6657126
ACGACAG657.9673366E-411.7022655
CGAAATC752.0793821E-411.39690213
GGACGTG8500.011.0638726
GACGTGA5000.010.8297927
AGGACGT8800.010.8046475
TGCACCG803.7417107E-410.69665
GTCCTAC6900.010.6170381
GTATAGG1803.45608E-1110.5710761
AGGACCG1002.376484E-510.4588995
CGGGAGT1002.3801704E-510.457434
TAGGACG9200.010.4367634
TAGGACA3750.010.3940524
TAGGACC2750.010.3710054
TTAAGAC1752.3283064E-1010.3216193
TAAGACT2400.010.2989844
TTAGGAC6050.010.2138693
GTATAGA2053.6379788E-1210.2101141
ACGTGAA5850.010.06798
CTAGGTT951.6323794E-410.007114
Produced by FastQC (version 0.11.5)