FastQCFastQC Report
Thu 26 May 2016
SRR1512764_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512764_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2054497
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT51660.2514484080531634No Hit
GTACATGGGAAGCAGTGGTATCAAC40430.19678782689874944No Hit
GTCCTACAGTGGACATTTCTAAATT36660.17843783660915544No Hit
GTCCTAAAGTGTGTATTTCTCATTT34230.16661012403522615No Hit
CTGTAGGACGTGGAATATGGCAAGA32420.15780018174764918No Hit
GGTATCAACGCAGAGTACTTTTTTT30640.14913626060296023No Hit
CTTTAGGACGTGAAATATGGCGAGG29920.14563175317364785No Hit
TATCAACGCAGAGTACTTTTTTTTT27190.13234382917083842No Hit
CCCATGTACTCTGCGTTGATACCAC26810.1304942280275902No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCCGT500.001504265713.2947876
TAGGACC6650.011.8527854
AAGCGTG855.3458265E-511.172097
TGGACGG803.7854532E-410.68331055
GTGTAGG3350.010.6298361
TGTAGGA21500.010.4248412
GTCTTAA2550.010.1904471
TGGCGAG19700.010.16790118
CTGTAGG19600.010.1644511
GAAGCGT856.61864E-410.054886
GTAGGAC20500.010.0531423
GTCCTAA10550.010.0348271
GTACCGT951.6509136E-49.9960796
GGACGTG21100.09.9913436
GGCGAGA12450.09.83855119
AGGACGT21350.09.785395
CGTGGAA11300.09.7278949
GTCTTAT2303.6379788E-129.62431051
GACTAAC909.888853E-49.62431051
ACCTTTT5850.09.58213615
Produced by FastQC (version 0.11.5)