FastQCFastQC Report
Thu 26 May 2016
SRR1512764_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512764_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2054497
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT61430.29900262692036056No Hit
GTACATGGGAAGCAGTGGTATCAAC44860.21835028233187975No Hit
GTCCTACAGTGGACATTTCTAAATT39450.1920178028977409No Hit
GGTATCAACGCAGAGTACTTTTTTT37760.18379194518171602No Hit
GTCCTAAAGTGTGTATTTCTCATTT37100.18057948003817964No Hit
CTGTAGGACGTGGAATATGGCAAGA36560.17795109946619536No Hit
TATCAACGCAGAGTACTTTTTTTTT35770.17410587603681096No Hit
CTTTAGGACGTGAAATATGGCGAGG33310.16213214231999365No Hit
CCCATGTACTCTGCGTTGATACCAC30490.14840615488852016No Hit
GTCCTACAGTGTGCATTTCTCATTT21410.10421042230774735No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC5500.013.4833384
GCACCGT905.42168E-712.6639886
TAAGACG803.7444435E-410.6959184
CCAACGA1252.2587665E-710.63386619
CTGGACG909.441892E-510.5638694
TAGGACT3650.010.419164
GGCGAGG8950.010.39623719
TGTAGGA22250.010.3419842
GAACAGT3600.010.2894916
CCCGTTT1201.5282621E-610.2894912
AATGTCC5450.010.2822298
GGACGTG19100.010.1941796
AGGACGT19500.010.1415625
GACGTGA10400.010.13727957
TTAGGAC12100.010.0575023
AGGACCT13050.010.0541355
CTGTAGG21100.09.9677921
GTAGGAC20200.09.9310833
GTATAAG2400.09.9132931
TGGCGAG20150.09.75367918
Produced by FastQC (version 0.11.5)