FastQCFastQC Report
Thu 26 May 2016
SRR1512763_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512763_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1848201
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT37030.20035699580294566No Hit
GTCCTACAGTGGACATTTCTAAATT23710.12828691251654986No Hit
CTGTAGGACGTGGAATATGGCAAGA22370.12103661885260317No Hit
GGTATCAACGCAGAGTACTTTTTTT22270.12049555216126384No Hit
GTCCTAAAGTGTGTATTTCTCATTT21870.11833128539590662No Hit
GTACATGGGAAGCAGTGGTATCAAC20150.1090249383048705No Hit
TATCAACGCAGAGTACTTTTTTTTT19950.1079428049221919No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCGC250.00605274718.9889746
GTCTTAT1950.011.837981
TAGGACC3800.011.2437764
AGGACGT13050.011.2042215
GGACGTG12950.011.0707916
GTAGGAC14350.010.9840663
TAGGACG13850.010.96865754
TGTAGGA15600.010.773472
AGGATCG803.7916627E-410.6812965
GACGTGG6900.010.5955857
TATGTCG909.4830175E-510.55920416
GTATAAT1854.7293724E-1110.3982261
GTATTAG1303.240766E-710.35823251
TGTACCG1106.088012E-610.3576215
GACGTGA6800.010.1928877
TTAGGAC8000.09.9700253
TAGACAG3050.09.9614295
GTGTAGA3300.09.9098241
TGGCGAG14350.09.85865518
ACGTGGA7300.09.8508768
Produced by FastQC (version 0.11.5)