FastQCFastQC Report
Thu 26 May 2016
SRR1512763_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512763_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1848201
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT45560.24650998457418863No Hit
GGTATCAACGCAGAGTACTTTTTTT27740.15009190017752397No Hit
GTCCTACAGTGGACATTTCTAAATT26320.1424087531605058No Hit
TATCAACGCAGAGTACTTTTTTTTT25790.1395410996964075No Hit
CTGTAGGACGTGGAATATGGCAAGA25450.13770147294585383No Hit
GTACATGGGAAGCAGTGGTATCAAC22440.12141536553654066No Hit
GTCCTAAAGTGTGTATTTCTCATTT21930.11865592541071018No Hit
CTTTAGGACGTGAAATATGGCGAGG20760.1123254451220403No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACCGT500.001501490713.2979346
ATATCGA604.0980423E-412.6646996
GTCCTAA6100.011.8583071
TAGGACC5350.011.7295574
AGGCCGA700.001493566310.8554576
TGTACCG803.7401225E-410.6971355
GTCCTAC13100.010.6076811
ATTTCTA9850.010.31261115
TAGACGA750.002628252110.1424695
CGGTACA750.002629434510.1419194
TAAGCCC856.543048E-410.0673474
GTACCGT856.603619E-410.0572616
TGTAGGA14400.010.037092
CTAAATT9300.09.80015219
AGTGGAC11050.09.7991178
AGGACCT10600.09.7776755
TTGGACT2054.0017767E-119.7399524
ACATTTC10550.09.721765513
TCCTACA14700.09.702882
GTAGGAC13400.09.6496993
Produced by FastQC (version 0.11.5)