FastQCFastQC Report
Thu 26 May 2016
SRR1512762_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512762_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1877502
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT44390.2364311729095362No Hit
GTATCAACGCAGAGTACTTTTTTTT44210.23547245222641575No Hit
GTCCTAAAGTGTGTATTTCTCATTT42520.22647113025711824No Hit
CTGTAGGACGTGGAATATGGCAAGA40030.2132088274739521No Hit
CTTTAGGACGTGAAATATGGCGAGG35370.18838861423316727No Hit
GTACATGGGAAGCAGTGGTATCAAC31020.16521953105775652No Hit
GTCCTACAGTGTGCATTTCTCATTT27860.1483886568429754No Hit
GGTATCAACGCAGAGTACTTTTTTT27520.14657773999708124No Hit
TATCAACGCAGAGTACTTTTTTTTT24370.12980012804247346No Hit
CCCATGTACTCTGCGTTGATACCAC20260.10790933911122333No Hit
CTGTAGGACCTGGAATATGGCGAGA18870.10050588494712656No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGCCA400.0053042514.24000812
TAGGACC9250.014.16721254
TGGCGAA759.764299E-713.92207218
TTGCCGG604.1207197E-412.65642818
AGTCGTC1603.6379788E-1211.8242068
AGGACCT17500.011.7752135
GGACCTG16750.011.4517546
GTGTAGG3700.011.4414841
GGCGAGG10050.011.42917919
GTAGGAC27250.011.1868773
TGTAGGA28150.011.0991022
TCCAACG1053.500567E-610.84836818
TGGCGAG25000.010.631418
ACGAAAT1701.4006218E-1010.61020312
GTATTAT2200.010.4959071
TAGAAAT6900.010.4595174
AATGTCC6450.010.4126348
GTGCTAT1303.2275238E-710.3613431
GCCGGTT1106.110622E-610.35442811
AAGTCGT1752.382876E-1010.3098177
Produced by FastQC (version 0.11.5)