FastQCFastQC Report
Thu 26 May 2016
SRR1512762_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512762_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1877502
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT54000.2876162049361332No Hit
GTCCTACAGTGGACATTTCTAAATT47520.25310226034379724No Hit
GTCCTAAAGTGTGTATTTCTCATTT46540.24788255884680815No Hit
CTGTAGGACGTGGAATATGGCAAGA44500.2370170577714431No Hit
CTTTAGGACGTGAAATATGGCGAGG42140.2244471643705306No Hit
GTACATGGGAAGCAGTGGTATCAAC34400.18322217499635154No Hit
GGTATCAACGCAGAGTACTTTTTTT32890.17517957371017445No Hit
TATCAACGCAGAGTACTTTTTTTTT31470.16761633276555762No Hit
GTCCTACAGTGTGCATTTCTCATTT29960.1595737314793806No Hit
CCCATGTACTCTGCGTTGATACCAC23420.12474021332600443No Hit
CTGAAGGACCTGGAATATGGCGAGA22400.11930746278832192No Hit
CTGTAGGACCTGGAATATGGCGAGA21620.11515300649480001No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGT400.00528539414.2476626
GCACCGT551.9632246E-413.8159136
TAGGACC9250.013.0539744
GTACCGT701.0928057E-412.2122816
ACCGTGC701.0930776E-412.2119578
TCCAACG1405.2750693E-1112.20675218
CCAACGT701.09743574E-412.20675219
AGGACCT17800.011.7515475
GGACCTG16900.011.2407596
GTCTTAC1950.011.2251241
CCTATAC1201.2611781E-711.0924523
GCCGGTT1201.2789678E-711.0812211
GTCCTAT2800.010.8765191
GGCGAGG9800.010.85044619
ACCTTTT6450.010.598392515
TGTAGGA26700.010.39944652
CTGTAGG26200.010.2071141
GTAGGAC25850.010.1882583
CCTTTTT7000.010.17229316
TAGAAAT7150.010.106214
Produced by FastQC (version 0.11.5)