FastQCFastQC Report
Thu 26 May 2016
SRR1512761_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512761_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2057681
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT40210.19541415797686815No Hit
GTCCTACAGTGGACATTTCTAAATT26840.13043809997759614No Hit
GGTATCAACGCAGAGTACTTTTTTT24750.12028103481540628No Hit
CTGTAGGACGTGGAATATGGCAAGA24480.11896887807196549No Hit
GTCCTAAAGTGTGTATTTCTCATTT23490.11415763667934924No Hit
TATCAACGCAGAGTACTTTTTTTTT21240.10322299715067593No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG400.00529349614.2444585
CACCGTC751.4854226E-512.6614317
ACCGTCC604.2536267E-412.6089938
GTTCTAA2350.012.28891
TAGGACC5350.011.3603454
GTATACG600.005326222611.230691
TGTCGAG855.3670177E-511.16775318
ACCGTCT700.001549628310.8077078
CGCCACC1158.7917215E-710.74178714
GCACCGT1158.8612796E-710.7352176
TATGTCG803.7614544E-410.69066716
ACACCGT909.552751E-510.5517096
CGTGCGC909.6288415E-510.54345510
AGGACGT15150.010.3425115
GGACGTG14950.010.2905636
TCCAACG1502.4750989E-810.12542918
GTATTAC2002.0008883E-1110.107621
GTCGAGG951.6545359E-49.99391119
CTTAGAC2002.5465852E-119.9725853
GGCGAGG6150.09.88015919
Produced by FastQC (version 0.11.5)