FastQCFastQC Report
Thu 26 May 2016
SRR1512761_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512761_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2057681
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT48900.23764616575649966No Hit
GGTATCAACGCAGAGTACTTTTTTT31410.15264756782027925No Hit
GTCCTACAGTGGACATTTCTAAATT29330.1425391010559946No Hit
TATCAACGCAGAGTACTTTTTTTTT29210.14195592028113202No Hit
CTGTAGGACGTGGAATATGGCAAGA26820.1303409031817857No Hit
GTCCTAAAGTGTGTATTTCTCATTT25260.12275955310857223No Hit
CTTTAGGACGTGAAATATGGCGAGG23030.11192211037570936No Hit
GTACATGGGAAGCAGTGGTATCAAC21660.10526412986269496No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACCG400.005250842314.2618475
CTCGAAC1700.012.28699318
TCGAACT1650.012.08390319
ATACCGT600.00588616311.0809526
AATGCCG1900.010.99362718
CCTCGAA2250.010.97141717
TCCTATA2350.010.9252972
ATGCCGG1951.8189894E-1210.71173919
TCTACAC3000.010.45817853
TAGGACC5100.010.4395364
AGGACGT14350.010.137345
TCCAACG1606.2336767E-910.08790218
GTCCTAA8700.010.0641261
GGCGAGG6800.09.9133719
GGACGTG14250.09.8645466
GTCCTAC14050.09.8219811
TACACTG11700.09.7516895
GCCTCGA2550.09.68066216
GCGAGGA2750.09.6708839
CTACACT3750.09.63424
Produced by FastQC (version 0.11.5)