FastQCFastQC Report
Thu 26 May 2016
SRR1512760_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512760_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2052188
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT50300.2451042497081164No Hit
GGTATCAACGCAGAGTACTTTTTTT29750.1449672252249794No Hit
GTACATGGGAAGCAGTGGTATCAAC27520.13410077439298934No Hit
TATCAACGCAGAGTACTTTTTTTTT26900.13107960869082172No Hit
GTCCTACAGTGGACATTTCTAAATT22950.11183185945926981No Hit
GTCCTAAAGTGTGTATTTCTCATTT21040.1025247199574308No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGTT1400.014.914494511
TCCAACG1600.014.23970318
CCGGTTT1457.2759576E-1212.43806212
GGCGAGG5750.012.053040519
TAGGACC5600.011.8690394
CTAGGAC2250.010.9738823
TGGCGAG12200.010.97157518
CGGTTTC1701.4006218E-1010.61465913
ATCCCGT1106.1244464E-610.35259510
GGCTTAT1408.0355676E-810.3097181
TTAGGAC10050.010.2998253
GTCCTAA6850.010.2545321
GTAGGAC14550.010.247223
CCAACGA1303.876812E-710.22412919
TGCCGGT2151.8189894E-1210.15196310
TAGGACT2900.010.1500744
TAGGACG14500.010.084594
TGTAGGA15650.010.07312
CAGTGCG951.6820077E-49.9776119
GGACGTG14200.09.9640396
Produced by FastQC (version 0.11.5)