FastQCFastQC Report
Thu 26 May 2016
SRR1512760_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512760_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2052188
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT58860.2868158277896567No Hit
GGTATCAACGCAGAGTACTTTTTTT38450.18736100201346076No Hit
TATCAACGCAGAGTACTTTTTTTTT34810.1696238356329927No Hit
GTACATGGGAAGCAGTGGTATCAAC30800.15008371552703748No Hit
GTCCTACAGTGGACATTTCTAAATT25840.1259143899096964No Hit
CTGTAGGACGTGGAATATGGCAAGA24030.11709453519852958No Hit
GTCCTAAAGTGTGTATTTCTCATTT21250.10354801801784241No Hit
CCCATGTACTCTGCGTTGATACCAC21010.10237853452022913No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC5450.013.2579984
GGACCGT655.4630844E-513.1515516
CAGGTCG550.003074156612.088219
TCCAACG2200.012.08379218
ATACGGC658.060137E-411.68627915
ATAGGAC1803.6379788E-1211.0919453
TAGGACA4850.010.7815664
GGCGAGG6100.010.73957619
ACCGTGC803.7795707E-410.6851158
GTCCTAT2500.010.6615321
CCAACGT1252.2591303E-710.63373719
GTAGGAC14850.010.5637563
CAGCGTG1355.7079887E-810.55329
TAAGGTG2350.010.5190615
AGGACGT13750.010.5102345
GGACGTG13650.010.5073236
CGTTTCC1451.4555553E-810.47735414
GTCCTAC15550.010.4068711
TGGCGAG12050.010.32172218
AGGACCT10950.010.2456465
Produced by FastQC (version 0.11.5)