FastQCFastQC Report
Thu 26 May 2016
SRR1512759_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512759_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1761940
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT38160.2165794521947399No Hit
GTCCTACAGTGGACATTTCTAAATT33820.19194751240110333No Hit
GTCCTAAAGTGTGTATTTCTCATTT29890.16964255309488405No Hit
CTGTAGGACGTGGAATATGGCAAGA29170.16555614833649274No Hit
CTTTAGGACGTGAAATATGGCGAGG26220.14881323995141718No Hit
GTCCTACAGTGTGCATTTCTCATTT24410.13854047243379458No Hit
GGTATCAACGCAGAGTACTTTTTTT22900.12997037356550167No Hit
TATCAACGCAGAGTACTTTTTTTTT20190.11458960009988989No Hit
GTACATGGGAAGCAGTGGTATCAAC20080.11396528826180233No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC7450.013.0020184
TAAGACG550.003077016212.0865474
TCCAACG1501.4551915E-1112.02315118
GTGTAGG2750.011.1980291
AACCGTG600.00589321911.0790187
TTGCGAG600.00591329711.073956518
ACTGCGA600.00605805711.0380268
GGCGAGG9500.010.89264419
CCGTCCT700.001518310910.8340369
GTATAAA3150.010.6925621
GTCCTAC18850.010.6188211
GTCGAGG909.5810814E-510.54843119
TGTAGGA21050.010.5119452
TGGCGAG19400.010.47051518
GTAGAAC4650.010.4158933
AGGACGT19900.010.4029825
AATGTCC5550.010.3987588
CTAGGAC3200.010.3871733
GTCTTAG2800.010.3106851
AGGACCT12900.010.3060635
Produced by FastQC (version 0.11.5)