FastQCFastQC Report
Thu 26 May 2016
SRR1512759_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512759_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1761940
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT45370.2575002554002974No Hit
GTCCTACAGTGGACATTTCTAAATT36610.20778233083986966No Hit
GTCCTAAAGTGTGTATTTCTCATTT33050.1875773295344904No Hit
CTGTAGGACGTGGAATATGGCAAGA32790.18610168337173796No Hit
CTTTAGGACGTGAAATATGGCGAGG29530.1675993507156884No Hit
GGTATCAACGCAGAGTACTTTTTTT28930.16419401341702897No Hit
TATCAACGCAGAGTACTTTTTTTTT27390.1554536476838031No Hit
GTCCTACAGTGTGCATTTCTCATTT26490.15034564173581394No Hit
GTACATGGGAAGCAGTGGTATCAAC21460.12179756404871903No Hit
CTGTAGGACCTGGAATATGGCGAGA20770.11788142615526069No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG1900.012.99163118
TAGGACC6950.012.5863764
GGCGAGG7850.011.1265919
GATATAC4150.011.009311
GTGTTAC1408.101779E-910.8782481
CCGGTTT1053.47672E-610.854465512
TAGTACT2500.010.6491684
AGGACCT12250.010.6339475
CTACACT3150.010.5646514
CCAACGT909.578141E-510.54875919
CCAACGA1553.7016434E-910.41264719
TGTAGGA20700.010.3367482
GGACCTG12150.010.3190296
AGTACTG2600.010.2398765
TACTCCG750.00262839710.1423535
GTAGGAC20350.010.138953
CTGTAGG20600.09.9805281
AGGACGT19350.09.926145
GTCCTAA11100.09.8614751
ATTTAGA5900.09.8411261
Produced by FastQC (version 0.11.5)