FastQCFastQC Report
Thu 26 May 2016
SRR1512758_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512758_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2071275
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT49040.23676238065925576No Hit
GTCCTACAGTGGACATTTCTAAATT32610.15743925842778No Hit
GGTATCAACGCAGAGTACTTTTTTT29830.1440175737166721No Hit
CTGTAGGACGTGGAATATGGCAAGA28280.13653426029860835No Hit
GTCCTAAAGTGTGTATTTCTCATTT27650.13349265548997596No Hit
TATCAACGCAGAGTACTTTTTTTTT25880.12494719436096123No Hit
GTCCTACAGTGTGCATTTCTCATTT22270.10751831601308373No Hit
CTTTAGGACGTGAAATATGGCGAGG21930.1058768150052504No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC602.5799587E-514.2436027
GTCTTAC1800.013.8998421
GGCGAGG7450.012.6178819
TCCAACG1302.0008883E-1012.41387918
TAGGACC7500.011.7755694
TGGCGAG16650.010.94684318
GTCCTAA9300.010.8646411
AATCCCG2100.010.85174119
TAGGACA7250.010.7407874
TGTAGGA19000.010.5468022
GTAGGAC19050.010.5191193
AGGACGT17350.010.3443085
TTAGGAC12400.010.2630163
GGACGTG16950.010.2520316
CCAACGA1409.762152E-810.17350719
GTATAAA3800.010.129441
GTGTAGG3650.010.0184331
GACGTGA8950.09.9731747
GTATCAA22950.09.9374441
ATGGCGA18550.09.93664517
Produced by FastQC (version 0.11.5)