FastQCFastQC Report
Thu 26 May 2016
SRR1512758_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512758_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2071275
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT59660.2880351474333442No Hit
GGTATCAACGCAGAGTACTTTTTTT38340.18510337835391244No Hit
TATCAACGCAGAGTACTTTTTTTTT36220.1748681367756575No Hit
GTCCTACAGTGGACATTTCTAAATT36110.17433706292018203No Hit
CTGTAGGACGTGGAATATGGCAAGA32880.1587428033457653No Hit
GTCCTAAAGTGTGTATTTCTCATTT28600.13807920242362798No Hit
CTTTAGGACGTGAAATATGGCGAGG25750.12431956162267202No Hit
GTCCTACAGTGTGCATTTCTCATTT23780.11480851166552003No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCG500.001501652613.2978699
GCACCGT1155.333277E-912.3905176
CTGTCCG951.04233E-611.9980779
TAGGACC7400.011.9488234
GGCGAGG6850.011.64214419
GGTATCA13550.011.5196881
GTATAAG2650.011.1340211
TGTAGGA21650.011.0240762
GTCCTAC20200.010.6957371
GACGTGG9750.010.6190687
GGACGTG17750.010.596516
TCCAACG1451.4584657E-810.47601818
AGGACGT18250.010.4719625
CTGTAGG22100.010.4652631
TGGCGAG17100.010.38220918
GTAGGAC21300.010.3557673
AGGACCT13800.010.2660195
GTCCTAA8350.010.25869751
GGACCTG13450.010.2409856
CCAACGA1303.8766302E-710.22419119
Produced by FastQC (version 0.11.5)