FastQCFastQC Report
Thu 26 May 2016
SRR1512757_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512757_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1694340
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT41030.24215918882868845No Hit
GTCCTACAGTGGACATTTCTAAATT25490.15044206003517593No Hit
GGTATCAACGCAGAGTACTTTTTTT25110.14819929884202698No Hit
CTGTAGGACGTGGAATATGGCAAGA24030.14182513545097206No Hit
GTCCTAAAGTGTGTATTTCTCATTT22660.1337393911493561No Hit
TATCAACGCAGAGTACTTTTTTTTT22030.13002112917124073No Hit
CTTTAGGACGTGAAATATGGCGAGG20590.12152224464983415No Hit
GTACATGGGAAGCAGTGGTATCAAC20310.11986968377067178No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCAT701.1017405E-412.20147910
TAGGACC4450.011.9499724
TCCAACG855.3657932E-511.16773718
ACCGTGC855.5620796E-511.1284098
ATAGGAC1553.1468517E-1011.0278943
TAGGCCC803.7858757E-410.6829554
GTCCTAG2800.010.660011
GTATTAG1552.93403E-910.5601771
GTCCTAA7400.010.539191
GTCTTAG1751.8371793E-1010.4536881
CCGGTTT750.002665649910.125113512
GCCGGTT750.0026702510.12300911
GTATAAA2201.8189894E-1210.06605151
AATGCCG856.859982E-410.0155688
GGCGAGG7000.09.90204719
GTCCTAC13650.09.8752781
CTTAATC1752.590241E-99.7675633
TTAGGAC8300.09.7250663
CCTACAC2450.09.69004253
GTGTAGG3500.09.6283971
Produced by FastQC (version 0.11.5)