FastQCFastQC Report
Thu 26 May 2016
SRR1512757_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512757_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1694340
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT50360.29722487812363513No Hit
GGTATCAACGCAGAGTACTTTTTTT31460.18567701878017398No Hit
TATCAACGCAGAGTACTTTTTTTTT29920.17658793394478087No Hit
GTCCTACAGTGGACATTTCTAAATT27980.16513804785344147No Hit
CTGTAGGACGTGGAATATGGCAAGA27650.1631903868172858No Hit
GTCCTAAAGTGTGTATTTCTCATTT24680.14566143749188473No Hit
CTTTAGGACGTGAAATATGGCGAGG24010.14170709538817475No Hit
GTACATGGGAAGCAGTGGTATCAAC22740.13421155140054536No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC4750.012.6097994
GGCGAGG7100.011.366631519
CGTACAC700.001481878110.8655883
GTCCTAT1701.3278623E-1010.6397431
TAGAAAT5100.010.6259054
GGACGTG15300.010.1812976
GTGTTAC1502.3663233E-810.1544211
TGCACCG750.002629466410.1418145
ATTAGGC750.002630756510.1412153
AGGACGT15900.010.1059365
TGGCGAG16000.010.08788618
ACCGTGC856.6055683E-410.0568398
TACTAGC951.6292252E-410.0089622
AATCATG1901.0004442E-109.9983227
CCAACGA951.6536893E-49.99418919
AATGTCC5400.09.8499078
ATTTAGA5800.09.8480381
GTTCTAA1451.568369E-79.8480381
TAGTACT1554.0132363E-89.814084
CTTACAC1951.6189006E-109.7511683
Produced by FastQC (version 0.11.5)