FastQCFastQC Report
Thu 26 May 2016
SRR1512756_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512756_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1998403
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT35770.17899292585129226No Hit
GGTATCAACGCAGAGTACTTTTTTT21680.1084866265713172No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA350.002176757716.278687
TACCTCG604.107862E-412.6611957
GTCTTAT1853.6379788E-1210.9260161
TTTAGAC1659.349606E-1010.3599423
GTCCTAA2053.6379788E-1210.329591
GGTATCA13000.010.2916671
GTGTAGG2750.010.1503161
ACTAGAC856.61631E-410.0552383
CGTGCGC951.6595176E-49.99090210
TCTTATA2002.5465852E-119.9714452
GTGTTAG1451.3359931E-79.9572071
GTATTAG1752.05182E-99.9003081
GTATTAT2157.2759576E-129.8491441
GTACTAA1105.9236023E-59.6252991
ATAGGAC1606.671871E-89.49661353
GACAGTC2106.730261E-119.4958967
GTCCTAC3300.09.3336241
GTATAAT3000.09.3044571
TAGGACT2451.8189894E-129.3023364
TTAGACT1752.687193E-89.2248174
Produced by FastQC (version 0.11.5)