FastQCFastQC Report
Thu 26 May 2016
SRR1512756_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512756_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1998403
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT45690.22863256310163665No Hit
GGTATCAACGCAGAGTACTTTTTTT28260.14141291821519483No Hit
TATCAACGCAGAGTACTTTTTTTTT27240.13630884261082474No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG905.357215E-712.6764385
CTACACG951.0309213E-612.0089584
TAGACTG1803.6379788E-1211.0918845
CCGTGCG600.005883460411.0816099
CTAGTAC1158.73506E-710.7471463
GTAGGAC3200.010.69547753
GGTATCA14550.010.3996161
TCTATAC1659.1677066E-1010.3713723
TTATACC1201.5095229E-610.2993484
CTACCCT1409.5855285E-810.1861694
TCTTATA1701.5334081E-910.068352
GCACTAT1151.0054884E-59.9303951
CTACACT2201.6370905E-119.50709154
TAGGACT1502.619836E-79.50709154
TAGGACC2303.6379788E-129.5070914
TGTAGGA3850.09.3855042
TTAGACA1951.6589183E-99.263324
TAAGAGT1752.6373527E-89.235464
TCGAACT1451.7204111E-69.16685719
GTATTAC1356.697306E-69.1641621
Produced by FastQC (version 0.11.5)