FastQCFastQC Report
Thu 26 May 2016
SRR1512755_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512755_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1816719
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT40460.2227091806713091No Hit
GGTATCAACGCAGAGTACTTTTTTT25140.13838133470283515No Hit
TATCAACGCAGAGTACTTTTTTTTT21240.11691406320955525No Hit
GTCCTACAGTGGACATTTCTAAATT19800.10898768604280575No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC4550.012.73043254
AAGTCGT751.4867221E-512.6601667
GCCGGTT658.0910535E-411.68080511
TGTAGGA13200.011.366322
GTAGGAC12800.011.2763973
GGACGTG10950.011.01260956
AGGACGT11450.010.780495
TCCAACG1158.891948E-710.73213218
CTTATAC1355.72345E-810.5510163
GTATTAG1657.421477E-1010.4988091
TAATACA2450.010.4645944
AGTCGTC1002.511749E-510.4065798
TAGGACG12050.010.4018754
GTCCTAC9400.010.3405821
CCGTGCC1201.5685073E-610.268469
GTCTAGC750.002377892110.2655021
AATCCCG1303.8670623E-710.225800519
GACGTGG5850.010.2255187
GACGTGA6150.010.1898897
ACCGAGG750.00274097210.0912288
Produced by FastQC (version 0.11.5)