FastQCFastQC Report
Thu 26 May 2016
SRR1512755_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512755_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1816719
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT44620.24560760359747433No Hit
GGTATCAACGCAGAGTACTTTTTTT28090.15461939903749564No Hit
TATCAACGCAGAGTACTTTTTTTTT26380.14520682615198058No Hit
GTCCTACAGTGGACATTTCTAAATT21880.12043689750588836No Hit
CTGTAGGACGTGGAATATGGCAAGA19350.1065106931781965No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACCGT954.9112714E-913.9981136
TCCAACG954.945832E-913.991178518
CCAACGA852.7206625E-713.40331319
TAGGACC4750.013.0104284
GCGCCAC751.4843168E-512.66216913
TGTAGGA11450.011.0443962
CCGGTTT951.3628827E-510.99821512
GGACGGG700.001493290510.85567956
TCGAACT1408.434654E-910.85030119
GTAGGAC11450.010.794683
CACCGTG1502.1373125E-910.7652167
GTCCTAG2700.010.5739351
TGCGCCA909.531852E-510.55384312
TATGTCG909.579862E-510.54861416
TGGACTG2900.010.4914575
CGCCACC1002.412797E-510.44456514
CTGTAGG11950.010.4323591
CTCGAAC1409.7779775E-810.17215618
ACTGTTC4600.010.1179238
GACGTGG5850.010.0670197
Produced by FastQC (version 0.11.5)